期刊
MICROCHEMICAL JOURNAL
卷 183, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2022.107965
关键词
Chemical vapor generation; Atomic fluorescence spectrometry; L-Cysteine; Label-free
资金
- National Natural Science Foundation of China
- Sichuan Science and Technol- ogy Program
- [81603291]
- [82174273]
- [2022NSFSC1339]
A simple and label-free method was developed for hydrogen peroxide (H2O2) assay by chemical vapor generation-atomic fluorescence spectrometry. The method is based on the inhibition of the atomic fluorescence signal of As(III) by L-Cysteine (L-Cys) and the oxidation of L-Cys to cystine by H2O2 and I-. The proposed method has the advantages of simplicity and cost-effectiveness.
Hydrogen peroxide (H2O2) plays an essential role as a signal molecule in regulating diverse biological processes. It is of practical significance for accurately detecting the concentration of H2O2. In this work, a simple and label -free method was developed for H2O2 assay by chemical vapor generation-atomic fluorescence spectrometry (CVG-AFS). The assay was based on the phenomenon that L-Cysteine (L-Cys) can inhibit the AFS signal of As(III), while L-Cys can be oxidized to cystine by H2O2 and I-. Therefore, the concentration of H2O2 was proportion to the AFS signal. Under the optimal experimental conditions, a good linear range from 50 mu M-4 mM was obtained, and the limit of detection (LODs) and relative standard deviation (RSD, n = 7) were calculated to be 9.9 mu M and 0.4%, respectively. The proposed method has been successfully applied for H2O2 assay in medical disinfectants with good recoveries. It has the advantages of simple and cost-effective.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据