Microbial community profiling in bio-stimulated municipal solid waste for effective removal of organic pollutants containing endocrine disrupting chemicals

The study was aimed to improve the degradation of organic pollutants in municipal solid waste (MSW) through the bio-stimulation process. The results showed that the physico-chemical properties of MSW (control) had a high value of pH (9.2 +/- 0.02); total suspended solids (TSS: 1547 +/- 23 mg/kg(-1)), and total dissolved solids (TDS:76 +/- 0.67 mg/kg(-1)). After the biostimulation process (biostimulated MSW), the physico-chemical pa-rameters of MSW were reduced as pH (7.1 +/- 0.01); TSS (41 +/- 0.01 mg/kg(-1)), and TDS (789 +/- 03 mg/kg(-1)). Furthermore, the major organic pollutants detected from MSW by gas chromatography-mass spectroscopy (GC -MS) analysis at different retention time (RT) were hexadecane (RT-8.79); pentadecane (RT-9.36); and hex-asiloxane (RT-9.43) while these organic pollutants were degraded after the biostimulation process. The whole-genome metagenome sequencing size (%) analyses showed major groups of bacteria (40.82%) followed by fungi (0.05%), virus (0.0032%), and archaea (0.0442%) in MSW. The species richness and evenness of the microbial community were decreased substantially due to the biostimulation treatment. The total number of genes in the biostimulated MSW (PS-3_11267) sample were 465302 whereas the number of genes in the control MSW (PS-4_11268) sample were 256807. Furthermore, the biostimulated MSW (PS-3_11267) aligned the reads to bacteria (19502525), fungi (40030), virus (3339), and archaea (12759) genomes whereas the control sample (PS-4_11268) aligned the reads to bacteria (17057259), fungi (19148), virus (1335), and archaea (18447) ge-nomes. Moreover, the relative abundance at genus level in biostimulated MSW (PS-3_11267) (Ochrobactrum and Phenylobacterium), phylum; (Proteobacteria and Actinobacteria), and species (Chthoniobacter flavus and Vulgati-bacter incomptus) level was the most abundant. The results provided valuable information regarding the degra-dation of organic pollutants in MSW by microbial communities through biostimulation for the prevention of soil pollution and health protection.

Automated summary

This study aimed to improve the degradation of organic pollutants in municipal solid waste (MSW) through the bio-stimulation process. The results showed that the physico-chemical properties of MSW were improved and major organic pollutants were degraded after the biostimulation process. These findings provide valuable information for preventing soil pollution and protecting health.