Deciphering pH-dependent microbial taxa and functional gene co-occurrence in the coral Galaxea fascicularis

How the coral microbiome responds to oceanic pH changes due to anthropogenic climate change, including ocean acidification and deliberate artificial alkalization, remains an open question. Here, we applied a 16S profile and GeoChip approach to microbial taxonomic and gene functional landscapes in the coral Galaxea fascicularis under three pH levels (7.85, 8.15, and 8.45) and tested the influence of pH changes on the cell growth of several coral-associated strains and bacterial populations. Statistical analysis of GeoChip-based data suggested that both ocean acidification and alkalization destabilized functional cores related to aromatic degradation, carbon degradation, carbon fixation, stress response, and antibiotic biosynthesis in the microbiome, which are related to holobiont carbon cycling and health. The taxonomic analysis revealed that bacterial species richness was not significantly different among the three pH treatments, but the community compositions were significantly distinct. Acute seawater alkalization leads to an increase in pathogens as well as a stronger taxonomic shift than acidification, which is worth considering when using artificial ocean alkalization to protect coral ecosystems from ocean acidification. In addition, our co-occurrence network analysis reflected microbial community and functional shifts in response to pH change cues, which will further help to understand the functional ecological role of the microbiome in coral resilience.

Automated summary

This study investigates the response of the coral microbiome to oceanic pH changes caused by anthropogenic climate change. The results show that both ocean acidification and alkalization disrupt the functional cores of the microbiome, affecting the carbon cycling and health of the coral holobiont. While bacterial species richness does not vary significantly among pH treatments, the community compositions show significant differences. Seawater alkalization leads to a stronger taxonomic shift and an increase in pathogens compared to acidification. The study highlights the importance of understanding the functional ecological role of the microbiome in coral resilience.