4.7 Article

CRISPRi-microfluidics screening enables genome-scale target identification for high-titer protein production and secretion

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Review Biotechnology & Applied Microbiology

Microbial protein cell factories fight back?

Lukas A. Rettenbacher et al.

Summary: The biopharmaceutical market is experiencing rapid growth, with mammalian cells and microorganisms competing for market dominance. While mammalian cells have been favored in antibody-based therapies, extensive research has increased our understanding of microbes and emerging therapeutic molecules are making them more viable. This review compares four microbial production hosts and explores their secretion and post-translational modification capabilities, as well as discussing the impact of tools like omics and systems biology, alternative production systems, and emerging biotherapeutics.

TRENDS IN BIOTECHNOLOGY (2022)

Article Multidisciplinary Sciences

CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction

Jiao Liu et al.

Summary: In this study, the authors use CRISPR technology and CRISPRi screening to convert a wild-type Corynebacterium glutamicum to a hyperproducer of L-proline, an amino acid with various applications. By optimizing the biosynthetic enzyme and regulating the carbon flux, they achieved efficient production of L-proline.

NATURE COMMUNICATIONS (2022)

Review Genetics & Heredity

A new era in functional genomics screens

Laralynne Przybyla et al.

Summary: The latest approaches in CRISPR-based functional genomics screens provide a scalable and multiplexable way to characterize genes and gene regulatory elements encoded by the human genome. These tools promise to reveal mechanisms of gene function and regulation, as well as enable exploration of how genes work together to modulate complex phenotypes.

NATURE REVIEWS GENETICS (2022)

Article Biotechnology & Applied Microbiology

Development of a novel platform for recombinant protein production in Corynebacterium glutamicum on ethanol

Xinyu Yu et al.

Summary: This study presents a novel recombinant protein manufacturing platform that utilizes ethanol as both a delayed carbon source and an inducer. By improving the inducible promoter and optimizing transcriptional engineering, this platform achieves superior protein expression in Corynebacterium glutamicum. Ethanol metabolism is found to be required for the upregulation of the improved promoter. The platform successfully produces the first secretory overexpression of the de novo design protein NEO-2/15.

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2022)

Article Biochemical Research Methods

Engineering a Supersecreting Strain of Escherichia coli by Directed Coevolution of the Multiprotein Tat Translocation Machinery

May N. Taw et al.

Summary: The study explores the isolation of supersecreting mutant Tat translocases through a directed coevolution strategy, demonstrating their ability to efficiently export heterologous proteins into the periplasm. These evolved translocases showed higher secretion levels compared to wild-type translocases, with quality control suppression being a common feature among them.

ACS SYNTHETIC BIOLOGY (2021)

Article Biotechnology & Applied Microbiology

Droplet-Based Microfluidic High Throughput Screening of Corynebacterium glutamicum for Efficient Heterologous Protein Production and Secretion

Suvasini Balasubramanian et al.

Summary: This study focuses on improving protein production and secretion in Corynebacterium glutamicum using droplet-based microfluidic high throughput screening. Through chemical mutagenesis and DBM screening, a library of C. glutamicum secreting beta-glucosidase was generated with several mutants showing enhanced enzyme secretion capacity. Whole-genome analysis revealed specific mutations in genes associated with protein synthesis and secretion, leading to higher secretion levels of beta-glucosidase and alpha-amylase in the supernatant of the mutants. These results demonstrate the potential of DBM screening as a powerful tool for optimizing cell factories for heterologous protein production.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2021)

Article Biotechnology & Applied Microbiology

Enhancement of heterologous protein production in Corynebacterium glutamicum via atmospheric and room temperature plasma mutagenesis and high-throughput screening

Lihong Meng et al.

Summary: The study utilized the ARTP mutagenesis and high-throughput screening strategy to engineer Corynebacterium glutamicum for high yield production of heterologous proteins. The research confirmed the growth stability of mutant strains and their potential for producing high-value medicinal proteins, leading to a significant increase in protein yield. Through systematic genome analysis, mutations in key genes were identified, providing insights for further research on optimizing protein production.

JOURNAL OF BIOTECHNOLOGY (2021)

Article Biochemistry & Molecular Biology

Development of a base editor for protein evolution via in situ mutation in vivo

Wenliang Hao et al.

Summary: The development of a base editor in Bacillus subtilis allows for efficient protein evolution in vivo. The base editor can introduce mutations with high efficiency, regulate conversion efficiency by changing culture conditions, and is applicable for constructing mutant protein libraries in vivo.

NUCLEIC ACIDS RESEARCH (2021)

Article Multidisciplinary Sciences

Genome-scale target identification in Escherichia coli for high-titer production of free fatty acids

Lixia Fang et al.

Summary: The study successfully identified gene targets for high-titer production of free fatty acids in E. coli using CRISPRi and omics analyses. By combinatorial perturbation of beneficial genes, a recombinant strain was engineered to produce 30.0 g L-1 FFAs, the highest reported titer in E. coli to date, demonstrating the potential for rewiring cellular metabolism to facilitate biochemical production.

NATURE COMMUNICATIONS (2021)

Review Biotechnology & Applied Microbiology

High-Throughput Screening Technology in Industrial Biotechnology

Weizhu Zeng et al.

TRENDS IN BIOTECHNOLOGY (2020)

Article Food Science & Technology

Recent Advances in Recombinant Protein Production by Bacillus subtilis

Kang Zhang et al.

Annual Review of Food Science and Technology (2020)

Article Biotechnology & Applied Microbiology

Omics Analysis Reveals the Mechanism of Enhanced Recombinant Protein Production Under Simulated Microgravity

Huangfu Jie et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)

Article Biochemistry & Molecular Biology

On-target activity predictions enable improved CRISPR-dCas9 screens in bacteria

Alicia Calvo-Villamanan et al.

NUCLEIC ACIDS RESEARCH (2020)

Review Microbiology

Translation elongation factor P (EF-P)

Katherine R. Hummels et al.

FEMS MICROBIOLOGY REVIEWS (2020)

Review Biochemistry & Molecular Biology

Redox regulation by reversible protein S-thiolation in Gram-positive bacteria

Marcel Imber et al.

REDOX BIOLOGY (2019)

Article Biotechnology & Applied Microbiology

The osnR gene of Corynebacterium glutamicum plays a negative regulatory role in oxidative stress responses

Haeri Jeong et al.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2019)

Article Multidisciplinary Sciences

Multi-functional genome-wide CRISPR system for high throughput genotype-phenotype mapping

Jiazhang Lian et al.

NATURE COMMUNICATIONS (2019)

Article Biotechnology & Applied Microbiology

Enhanced extracellular pullulanase production in Bacillus subtilis using protease-deficient strains and optimal feeding

Kang Zhang et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2018)

Article Biotechnology & Applied Microbiology

Rapid identification of unknown carboxyl esterase activity in Corynebacterium glutamicum using RNA-guided CRISPR interference

Seung Soo Lee et al.

ENZYME AND MICROBIAL TECHNOLOGY (2018)

Article Biochemistry & Molecular Biology

CosR is an oxidative stress sensing a MarR-type transcriptional repressor in Corynebacterium glutamicum

Meiru Si et al.

BIOCHEMICAL JOURNAL (2018)

Article Genetics & Heredity

Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors

Francois Rousset et al.

PLOS GENETICS (2018)

Review Microbiology

Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application

Min Ju Lee et al.

FRONTIERS IN MICROBIOLOGY (2018)

Article Biotechnology & Applied Microbiology

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering

Andrew D. Garst et al.

NATURE BIOTECHNOLOGY (2017)

Article Agriculture, Multidisciplinary

High-Efficiency Secretion of β-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization

Yafeng Song et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2017)

Article Biotechnology & Applied Microbiology

Assessing glycolytic flux alterations resulting from genetic perturbations in E. coli using a biosensor

Christina E. Lehning et al.

METABOLIC ENGINEERING (2017)

Review Microbiology

Oxidative stress, protein damage and repair in bacteria

Benjamin Ezraty et al.

NATURE REVIEWS MICROBIOLOGY (2017)

Article Multidisciplinary Sciences

Efficient protein production by yeast requires global tuning of metabolism

Mingtao Huang et al.

NATURE COMMUNICATIONS (2017)

Review Biotechnology & Applied Microbiology

Enabling tools for high-throughput detection of metabolites: Metabolic engineering and directed evolution applications

Jyun-Liang Lin et al.

BIOTECHNOLOGY ADVANCES (2017)

Review Chemistry, Multidisciplinary

Nonribosomal Peptide Synthesis-Principles and Prospects

Roderich D. Suessmuth et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

Review Biotechnology & Applied Microbiology

Expression of recombinant protein using Corynebacterium Glutamicum: progress, challenges and applications

Xiuxia Liu et al.

CRITICAL REVIEWS IN BIOTECHNOLOGY (2016)

Article Biochemistry & Molecular Biology

A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria

Jason M. Peters et al.

Article Biotechnology & Applied Microbiology

Mycothiol protects Corynebacterium glutamicum against acid stress via maintaining intracellular pH homeostasis, scavenging ROS, and S-mycothiolating MetE

Yingbao Liu et al.

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY (2016)

Article Multidisciplinary Sciences

The anti-sigma factor RsrA responds to oxidative stress by reburying its hydrophobic core

Karthik V. Rajasekar et al.

Nature Communications (2016)

Article Biochemical Research Methods

Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi)

Sara Cleto et al.

ACS SYNTHETIC BIOLOGY (2016)

Article Biochemistry & Molecular Biology

ABC transporter research: going strong 40 years on

Frederica L. Theodoulou et al.

BIOCHEMICAL SOCIETY TRANSACTIONS (2015)

Article Biochemistry & Molecular Biology

The substrate-binding protein in bacterial ABC transporters: dissecting roles in the evolution of substrate specificity

Abbas Maqbool et al.

BIOCHEMICAL SOCIETY TRANSACTIONS (2015)

Article Biotechnology & Applied Microbiology

Biosensor-driven adaptive laboratory evolution of L-valine production in Corynebacterium glutamicum

Regina Mahr et al.

METABOLIC ENGINEERING (2015)

Article Multidisciplinary Sciences

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Silvana Konermann et al.

NATURE (2015)

Article Multidisciplinary Sciences

Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast

Mingtao Huang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Biotechnology & Applied Microbiology

High-level secretory production of recombinant single-chain variable fragment (scFv) in Corynebacterium glutamicum

Sung Sun Yim et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Beyond Growth Rate 0.6: What Drives Corynebacterium glutamicum to Higher Growth Rates in Defined Medium

Simon Unthan et al.

BIOTECHNOLOGY AND BIOENGINEERING (2014)

Article Biochemistry & Molecular Biology

Droplet-Based Microfluidics Platform for Ultra-High-Throughput Bioprospecting of Cellulolytic Microorganisms

Majdi Najah et al.

CHEMISTRY & BIOLOGY (2014)

Article Biochemical Research Methods

High-throughput screening for industrial enzyme production hosts by droplet microfluidics

Staffan L. Sjostrom et al.

LAB ON A CHIP (2014)

Article Biochemical Research Methods

Universal Genetic Assay for Engineering Extracellular Protein Expression

Charles H. Haitjema et al.

ACS SYNTHETIC BIOLOGY (2014)

Article Biotechnology & Applied Microbiology

Isolation of Fully Synthetic Promoters for High-Level Gene Expression in Corynebacterium glutamicum

Sung Sun Yim et al.

BIOTECHNOLOGY AND BIOENGINEERING (2013)

Article Biochemistry & Molecular Biology

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

David Bikard et al.

NUCLEIC ACIDS RESEARCH (2013)

Article Biochemical Research Methods

FLASH: fast length adjustment of short reads to improve genome assemblies

Tanja Magoc et al.

BIOINFORMATICS (2011)

Review Biotechnology & Applied Microbiology

Sigma factors and promoters in Corynebacterium glutamicum

Miroslav Patek et al.

JOURNAL OF BIOTECHNOLOGY (2011)

Article Biochemistry & Molecular Biology

RosR (Cg1324), a Hydrogen Peroxide-sensitive MarR-type Transcriptional Regulator of Corynebacterium glutamicum

Michael Bussmann et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2010)

Review Biotechnology & Applied Microbiology

Applications of thiol-disulfide oxidoreductases for optimized in vivo production of functionally active proteins in Bacillus

Thijs R. H. M. Kouwen et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2009)

Article Biochemistry & Molecular Biology

Regulation of Quinone Oxidoreductase by the Redox-sensing Transcriptional Regulator QorR in Corynebacterium glutamicum

Shigeki Ehira et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2009)

Article Biochemical Research Methods

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

Jean-Christophe Baret et al.

LAB ON A CHIP (2009)

Article Biochemical Research Methods

Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources

Da Wei Huang et al.

NATURE PROTOCOLS (2009)

Article Multidisciplinary Sciences

Structure of an ABC transporter in complex with its binding protein

Kaspar Hollenstein et al.

NATURE (2007)

Article Biochemistry & Molecular Biology

The whcE gene of Corynebacterium glutamicum is important for survival following heat and oxidative stress

TH Kim et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2005)

Article Chemistry, Multidisciplinary

New biarsenical Ligands and tetracysteine motifs for protein labeling in vitro and in vivo: Synthesis and biological applications

SR Adams et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2002)