Enhancing the paracrine effects of adipose stem cells using nanofiber- based meshes prepared by light-welding for accelerating wound healing

The interaction between electrospinning nanomaterials and stem cells to repair damaged tissue mainly focus on manipulating cell viability, proliferation, or paracrine by the materials and their specific topology. In particular, the paracrine products of stem cells influence the behavior of those repairable cells (i.e., epidermal cells, fibroblasts, and endothelial cells) to speed up the repair progress. In this study, a series of nanofiber-based meshes were fabricated by electrospinning and light-welding technology to investigate their impacts on the paracrine behaviors of adipose stem cells (ADSCs) and their performance in accelerating wound healing. We found that the nanofiber-based meshes with varied grid sizes and modified with gelatin nanofibers could regulate the paracrine of ADSCs. As a result, the migration of fibroblasts and endothelial cells as well as angiogenesis was all influenced in vitro. In vivo investigation using rat skin wound healing models also indicated that the nanofiber-based meshes could accelerate wound healing rate, increase the thickness of the regenerated epidermis, and promote type I collagen production and CD31-positive vessel formation in the dermis layer. Taken together, such nanofiber-based meshes provide effective and promising alternatives to design tissue-repair scaffolds involving manipulating stem cell paracrine and wound closure. & COPY; 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Automated summary

The interaction between electrospinning nanomaterials and stem cells has been studied for repairing damaged tissue. The focus is on manipulating cell behavior through materials and their specific topology. Nanofiber-based meshes have been tested and found to regulate the paracrine of adipose stem cells, influencing migration and angiogenesis. In vivo investigations using rat skin wound healing models also showed promising results in wound closure and collagen production.