期刊
MARINE DRUGS
卷 20, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/md20120749
关键词
oyster; peptide; photoaging; purification; characterization
资金
- Modern Agricultural Industry Technology Research System of China
- Program of Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety [CARS-49]
- Guangdong Medical University [GDPKLAPPS2017]
- [4SG22196G]
This study purified and identified antiphotoaging peptides from oyster protein enzymatic hydrolysates and investigated their mechanism of action. The peptides exerted a protective effect on UVB-irradiated photoaged cells by inhibiting ROS production and regulating signaling pathways. The study highlights the importance of oyster protein enzymatic hydrolysates in preventing and regulating skin photoaging.
This study aimed to purify and identify antiphotoaging peptides from oyster (Crassostrea hongkongensis) protein enzymatic hydrolysates (OPEH) and to investigate the possible mechanism underlying its antiphotoaging effect. Multiple methods (Ultrafiltration, G25 Chromatography, RP-HPLC, and LC/MS/MS) had been used for this purpose, and eventually, two peptides, including WNLNP and RKNEVLGK, were identified. Particularly, WNLNP exerted remarkable antiphotoaging effect on the UVB-irradiated HaCaT photoaged cell model in a dose-dependent manner. WNLNP exerted its protective effect mainly through inhibiting ROS production, decreasing MMP-1 expression, but increasing extracellular pro-collagen I content. Furthermore, WNLNP downregulated p38, JNK, ERK, and p65 phosphorylation in the MAPK/NF-kappa B signaling pathway and attenuated bax over-expressions but reversed bcl-2 reduction in UVB- irradiated HaCaT cells. The molecular docking analysis showed that WNLNP forms five and seven hydrogen bonds with NF-kappa B (p65) and MMP-1, respectively. This study suggested that a pentapeptide WNLNP isolated from OPEH had great potential to prevent and regulate skin photoaging.
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