4.7 Article

Characterization of a Novel Alginate Lyase with Two Alginate Lyase Domains from the Marine Bacterium Vibrio sp. C42

期刊

MARINE DRUGS
卷 20, 期 12, 页码 -

出版社

MDPI
DOI: 10.3390/md20120746

关键词

alginate lyase; catalytic domain; alginate degradation; PL7 lyase; Vibrio; marine bacterium

资金

  1. Major Scientific and Technological Innovation Project (MSTIP) of Shandong Province
  2. Key R&D Program of Shandong Province [2019JZZY010817]
  3. National Science Foundation of China [2021CXGC010502]
  4. Fundamental Research Funds for the Central Universities [32170127, 42176156, 41906195]
  5. [202141006]

向作者/读者索取更多资源

In this study, a novel alginate lyase, AlyC8, with two functional catalytic domains (CD1 and CD2) was discovered. Both CD1 and CD2 showed synergistic effects in alginate degradation. The results demonstrated that CD1 plays a major role in the degradation of alginate by AlyC8, and CD1 and CD2 have different substrate specificities.
Alginate is abundant in the cell walls of brown algae. Alginate lyases can degrade alginate, and thus play an important role in the marine carbon cycle and industrial production. Currently, most reported alginate lyases contain only one functional alginate lyase domain. AlyC8 is a putative alginate lyase with two alginate lyase domains (CD1 and CD2) from the marine alginate-degrading strain Vibrio sp. C42. To characterize AlyC8 and its two catalytic domains, AlyC8 and its two catalytic domain-deleted mutants, AlyC8-CD1 and AlyC8-CD2, were expressed in Escherichia coli. All three proteins have noticeable activity toward sodium alginate and exhibit optimal activities at pH 8.0-9.0 and at 30-40 degrees C, demonstrating that both CD1 and CD2 are functional. However, CD1 and CD2 showed opposite substrate specificity. The differences in substrate specificity and degradation products of alginate between the mutants and AlyC8 demonstrate that CD1 and CD2 can act synergistically to enable AlyC8 to degrade various alginate substrates into smaller oligomeric products. Moreover, kinetic analysis indicated that AlyC8-CD1 plays a major role in the degradation of alginate by AlyC8. These results demonstrate that AlyC8 is a novel alginate lyase with two functional catalytic domains that are synergistic in alginate degradation, which is helpful for a better understanding of alginate lyases and alginate degradation.

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