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Recent Development of Brain Organoids for Biomedical Application

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Silver nanoparticles exposure induces developmental neurotoxicity in hiPSC-derived cerebral organoids

Yan Huang et al.

Summary: This study investigated the neurotoxic effects of silver nanoparticles (AgNPs) on cerebral organoids derived from induced pluripotent stem cells. The results showed that at low concentrations, AgNPs increased cell proliferation and inhibited neural apoptosis, but impaired cilium assembly and elongation, leading to abnormal organoid growth. At high concentrations, AgNPs significantly inhibited cell proliferation and induced apoptosis, disrupting the expression and co-localization of cytoskeleton proteins and perturbing neurite growth. Overall, AgNPs exposure can induce developmental neurotoxic effects in cerebral organoids and may be a potential congenital risk factor.

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X. Zheng et al.

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Juan Yin et al.

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Brain organoid formation on decellularized porcine brain ECM hydrogels

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Guini Song et al.

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High-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids

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Summary: By utilizing a robust method without the need for mouse co-culture, three-dimensional cerebellar organoids were successfully generated from human pluripotent stem cells (hPSCs), displaying hallmarks of in vivo cerebellar development. Single-cell profiling revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types. This xeno-free human cerebellar organoid model provides valuable insight into cerebellar development and associated disorders through enrichment of cerebellar disease genes in distinct cell populations.

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Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System

Yujin Ahn et al.

Summary: Recent studies have shown that blood vessel organoids (BVOs) can infiltrate human cerebral organoids and form a blood-brain barrier, creating neural-specific blood-vessel networks that can be maintained for over 50 days.
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Klotho inhibits neuronal senescence in human brain organoids

Mohammed R. Shaker et al.

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Robust production of uniform human cerebral organoids from pluripotent stem cells

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Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output

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