Dual-Function Fibrous Co-Polypeptide Scaffolds for Neural Tissue Engineering

This paper reports dual-function (high cell attachment and cell viability) fibrous scaffolds featuring aligned fibers, displaying good biocompatibility and no cytotoxicity. These scaffolds are fabricated through the electrospinning of a co-polypeptide comprising molar equivalents of N-6-carbobenzyloxy-l-lysine and gamma-benzyl-l-glutamate, with the lysine moieties enhancing cell adhesion and the neural-stimulating glutamate moieties improving cell viability. These new scaffolds allow neural cells to attach and grow effectively without any special surface treatment or coating. Pheochromocytoma (PC-12) cells grown on these scaffolds exhibit better neuronal activity and longer neurite length, relative to those grown on scaffolds prepared from their respective homo-polypeptides. When the scaffolds are partially hydrolyzed such that they present net positive charge and increased hydrophilicity, the cell viability and neurite growth both increase further. Accordingly, these novel co-polypeptide fibrous scaffolds have potential applications in neural tissue engineering.

Automated summary

This paper presents dual-function fibrous scaffolds that promote cell attachment and viability. The scaffolds are made from a co-polypeptide with lysine enhancing cell adhesion and glutamate improving cell viability. Neural cells grown on these scaffolds exhibit better activity and longer neurite length. Additionally, when the scaffolds are hydrolyzed, cell viability and neurite growth further increase.