Bovine serum albumin plays an important role in the removal of acrylamide by Lactobacillus strains

This study aimed to investigate the removal mechanism of acrylamide (AA) using a combination of Lactobacillus strains and Bovine Serum Albumin (BSA). A combination of 0.5% BSA with 1010 CFU/mL Lactobacillus strains substantially improved the AA removal rate. A 35.94% AA removal was observed for the combination strain 1611 with BSA and 30.89% for strain ML32 with BSA. The results indicated that increasing the surface hydro-phobicity of the two strains by adding BSA improved AA removal rates. The mechanism of the removal process was evaluated. Fourier Transform Infrared (FTIR) Spectroscopy analysis indicated that the original functional groups, C-O, N-H, and C=O, were involved in the removal of AA. According to the X-ray Diffraction (XRD) analysis, the unchanged crystalline structure of the cells proved that AA removal is a physical process. Scanning Electron Microscopy (SEM) images showed that the typical morphology and complete structure of AA-exposed cells were maintained. Energy Dispersive X-ray Spectroscopy (EDS) analysis demonstrated that surface pro-teins as well as amino components play an important role in AA removal. The zeta potential indicated that charge transfer was involved in AA removal. In general, AA removal by the combination of Lactobacillus strains with BSA can be considered an effective, physical removal method.

Automated summary

This study investigated the removal mechanism of acrylamide using a combination of Lactobacillus strains and Bovine Serum Albumin (BSA). The addition of 0.5% BSA and 1010 CFU/mL Lactobacillus strains significantly improved the removal rate of acrylamide. The results indicated that increasing the surface hydrophobicity of the strains by adding BSA improved the removal rates of acrylamide. Fourier Transform Infrared (FTIR) Spectroscopy analysis showed the involvement of original functional groups in the removal process, X-ray Diffraction (XRD) analysis demonstrated the physical nature of the removal process, Scanning Electron Microscopy (SEM) images showed the maintenance of cell morphology, and Energy Dispersive X-ray Spectroscopy (EDS) analysis showed the importance of surface proteins and amino components in acrylamide removal. Zeta potential indicated the involvement of charge transfer in the removal process. Overall, the combination of Lactobacillus strains with BSA can be considered an effective, physical removal method for acrylamide.