Transport Mechanism of Nicotine in Primary Cultured Alveolar Epithelial Cells

Nicotine is absorbed from the lungs into the systemic circulation during cigarette smoking. However, there is little information concerning the transport mechanism of nicotine in alveolar epithelial cells. In this study, we characterized the uptake of nicotine in rat primary cultured type II [TII] and trans-differentiated type I-like (TIL) epithelial cells. In both TIL and TIL cells, [H-3] nicotine uptake was time and temperature-dependent, and showed saturation kinetics. [H-3] Nicotine uptake in these cells was not affected by Na+, but was sensitive to extracellular and intracellular pH, suggesting the involvement of a nicotine/proton antiport system. The uptake of [H-3] nicotine in these cells was potently inhibited by organic cations such as clonidine, diphenhydramine, and pyrilamine, but was not affected by substrates and/or inhibitors of known organic cation transporters such as carnitine, 1-methyl-4-phenylpyridinium, and tetraethylammonium. In addition, the uptake of [H-3] nicotine in TIL cells was stimulated by pre-loading the cells with unlabeled nicotine, pyrilamine, and diphenhydramine, but not with tetraethylammonium. These results suggest that a novel proton-coupled antiporter is involved in the uptake of nicotine in alveolar epithelial cells and its absorption from the lungs into the systemic circulation. (C) 2016 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved.