Shear-enhanced sorting of ovoid and filamentous bacterial cells using pinch flow fractionation

In this paper, we experimentally investigate the influence of the flow rate on the trajectory of ovoid and filamentous bacterial cells of E. coli in a low aspect ratio pinch flow fractionation device. To that aim, we vary the Reynolds number over two orders of magnitude, while monitoring the dynamics of the cells across our device. At low flow rates, filamentous cells adopt several rotational motions in the pinched segment, which are induced both by the shear rate and by their close interactions with the nearest wall. As a result, the geometrical centre of the filamentous cells deviates towards the centre of the channel, which increases their effective sorting diameter depending on the length of their major axis as well as on the rotational mode they adopt in the pinch. As the flow rate increases, particles are forced to align vertically in the pinch, in the direction of the main shear gradient, which reduces the amplitude of the lateral deviation generated by their rotation. The trajectory of the particles in the expansion is directly determined by their position at the pinch outlet. As a consequence, the position of the filamentous cells at the outlet of the device strongly depends on the flow rate as well as on the length of their major axis. Based on these observations we optimized the flow conditions to successfully extract an ultra high purity sample of filamentous cells from a solution containing mainly ovoid cells.

Automated summary

In this paper, the influence of flow rate on the trajectory of Elliptical and filamentous bacterial cells of E. coli in a low aspect ratio pinch flow fractionation device is experimentally investigated. The results show that at low flow rates, filamentous cells exhibit multiple rotational motions in the pinched segment due to shear rate and their interactions with the nearest wall. As the flow rate increases, particles align vertically in the pinch, reducing the lateral deviation caused by rotation. The position of filamentous cells at the outlet depends on flow rate and the length of their major axis.