期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 125, 期 -, 页码 114-121出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2016.03.045
关键词
Non steroidal anti-inflammatory drugs (NSAIDs); Microextraction by packed sorbent (MEPS); Response surface methodology (RSM); Ultra-high performance liquid; chromatography (UHPLC) analysis; Dialyzed samples
A procedure based on microextraction by packed sorbent (MEPS) followed by ultra-high performance liquid chromatography (UHPLC) with photodiode array (PDA) detection has been developed for the analysis of seven selected non steroidal anti-inflammatory drugs (NSAIDs) in human dialysates. The influence on MEPS efficiency of pH of the sample, pH of the washing solvent and methanol content in the hydro-alcoholic elution mixture has been investigated by response surface methodology based on a Box-Behnken design of experiments. Among the above factors, pH of sample is the variable that mostly influences MEPS recovery. UHPLC separation of the NSAIDs was completed within less than 4 min under isocratic elution conditions on a Fortis SpeedCore C-18 column (150 x 4.6 mm I.D., 2.6 mu m) using acetonitrile-phosphate buffer as the mobile phase. Calibration curves of the NSAIDs were linear over the concentration range 0.025-15 mu g/mL, with correlation coefficients >= 0.998. Intra- and inter-assay relative standard deviations were <8% and recovery values ranged from 94% to 100% for the quality control samples. The results reveal that the developed MEPS/PDA-UHPLC method exhibits a good accuracy and precision and is well suited for the rapid analysis of human dialysate from patients treated with the selected NSAIDs. (C) 2016 Elsevier B.V. All rights reserved.
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