4.7 Review

Integration of CRISPR/Cas9 with artificial intelligence for improved cancer therapeutics

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Review Biochemistry & Molecular Biology

CRISPR-Cas9 gRNA efficiency prediction: an overview of predictive tools and the role of deep learning

Vasileios Konstantakos et al.

Summary: This article discusses the methods related to the on-target activity problem in CRISPR-Cas9 guide design and evaluates the performance of relevant tools. Suggestions for future challenges and directions are also provided.

NUCLEIC ACIDS RESEARCH (2022)

Review Oncology

CRISPR in cancer biology and therapy

Alyna Katti et al.

Summary: Over the past decade, CRISPR has played a crucial role in biomedical research, particularly in cancer research. It has provided new methods for studying cancer and improving diagnosis and treatment.

NATURE REVIEWS CANCER (2022)

Article Oncology

KAT7 is a genetic vulnerability of acute myeloid leukemias driven byMLLrearrangements

Yan Zi Au et al.

Summary: The study identifies the importance of KAT7 in AML, showing that its loss leads to the loss of related marks and affects proliferation and differentiation of AML cells. The acetyltransferase activity of KAT7 is crucial for cell proliferation, making it a potential therapeutic target for this subtype of AML with poor prognosis.

LEUKEMIA (2021)

Article Biotechnology & Applied Microbiology

enAsCas12a Enables CRISPR-Directed Evolution to Screen for Functional Drug Resistance Mutations in Sequences Inaccessible to SpCas9

Jasper Edgar Neggers et al.

Summary: This study demonstrates an enhanced AsCas12a variant that improves the activity and resolution in screening for drug resistance mutations in regions not accessible to other CRISPR nucleases. This approach can be used for identifying drug targets and binding sites, leading to the discovery of new drug resistance mutations against inhibitors of NAMPT and KIF11.

MOLECULAR THERAPY (2021)

Article Multidisciplinary Sciences

A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells

Kazuhiro Murakami et al.

Summary: Safe utilization of the regenerative potential of adult stem cells for clinical applications requires a comprehensive understanding of the regulatory mechanisms. Epithelial organoid cultures accurately mimic in vivo stem cell-driven epithelial renewal, offering a great ex vivo platform for studying key regulatory mechanisms. Through genome-scale CRISPR knockout screening, Alk, Bclaf3, and Prkra were identified as potential regulators of gastric epithelial differentiation and could suppress stemness/proliferation.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Immunology

An NR2F1-specific agonist suppresses metastasis by inducing cancer cell dormancy

Bassem D. Khalil et al.

JOURNAL OF EXPERIMENTAL MEDICINE (2021)

Article Cell Biology

Genome-Scale CRISPR-Cas9 Transcriptional Activation Screening in Metformin Resistance Related Gene of Prostate Cancer

Jiahong Chen et al.

Summary: The study identified that activation of ECE1, ABCA12, BPY2, EEF1A1, RAD9A, and NIPSNAP1 genes can lead to resistance of prostate cancer cells to metformin, with RAD9A playing the most significant role in resistance. High levels of RAD9A may upregulate regulatory T cells in the tumor immune microenvironment to counterbalance the effects of metformin.

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (2021)

Article Biochemistry & Molecular Biology

Massively parallel assessment of human variants with base editor screens

Ruth E. Hanna et al.

Summary: Understanding the functional consequences of single-nucleotide variants is crucial for uncovering the genetic basis of diseases. Leveraging CRISPR-Cas9 cytosine base editors, this study demonstrates a scalable approach to assay variants in mammalian cells. The use of base editor screens allows for identification of mutations conferring drug sensitivity or resistance, as well as functionalization of genetic variants through sgRNA libraries.
Article Biochemistry & Molecular Biology

Functional interrogation of DNA damage response variants with base editing screens

Raquel Cuella-Martin et al.

Summary: Through CRISPR-dependent cytosine base editing screens, this study identified over 2,000 sgRNAs that induce nucleotide variants in DNA damage response (DDR) genes, altering cellular fitness upon DNA damage. The research also discovered critical mutations in DDR regulator 53BP1 and TRAIP ligase, shedding light on their roles in cellular damage response. The findings provide valuable insights into the function of DDR genes and their implications in human diseases.
Review Biochemistry & Molecular Biology

Application of the CRISPR/Cas9-based gene editing technique in basic research, diagnosis, and therapy of cancer

Huimin Zhang et al.

Summary: The 2020 Nobel Prize in Chemistry was awarded to Emmanuelle Charpentier and Jennifer Doudna for their development of CRISPR/Cas9 gene editing technology, which has provided new tools for precise gene editing. This technology has been shown to be increasingly used in cancer research and treatment, achieving remarkable results in exploring tumorigenesis and development mechanisms.

MOLECULAR CANCER (2021)

Article Genetics & Heredity

Multimodal pooled Perturb-CITE-seq screens in patient models define mechanisms of cancer immune evasion

Chris J. Frangieh et al.

Summary: The study developed a novel method Perturb-CITE-seq, which combines single-cell transcriptome and protein readouts to uncover new mechanisms associated with cancer cell-intrinsic ICI resistance, such as the loss of CD58 leading to immune evasion.

NATURE GENETICS (2021)

Article Medicine, Research & Experimental

Targeted genome editing in vivo corrects a Dmd duplication restoring wild-type dystrophin expression

Eleonora Maino et al.

Summary: Tandem duplication mutations are increasingly being recognized as a direct cause of rare heritable diseases, but the lack of suitable animal models has hindered research and therapeutic development. A novel mouse model with a multi-exonic tandem duplication in the Dmd gene was successfully corrected using a CRISPR/Cas9 approach, leading to restoration of full-length dystrophin expression and improvements in histopathological and clinical phenotypes. This study highlights the potential of CRISPR/Cas9 as a powerful tool for accurately modeling and treating tandem duplication mutations.

EMBO MOLECULAR MEDICINE (2021)

Review Oncology

Chimeric antigen receptor natural killer (CAR-NK) cell design and engineering for cancer therapy

Ying Gong et al.

Summary: NK cells, especially CAR-NK cells, play a crucial role in cancer treatment. Advances in CAR-NK technology show promising potential in efficiently targeting cancer cells with reduced side effects. These developments contribute to improving cancer treatment strategies.

JOURNAL OF HEMATOLOGY & ONCOLOGY (2021)

Review Oncology

CAR-T cell therapy: current limitations and potential strategies

Robert C. Sterner et al.

Summary: CAR-T cell therapy has shown remarkable clinical responses in certain subsets of leukemia or lymphoma, but faces challenges in solid tumors and hematological malignancies. Innovative strategies and approaches are necessary to overcome these challenges and improve the efficacy of CAR-T cells.

BLOOD CANCER JOURNAL (2021)

Review Immunology

CRISPR-Based COVID-19 Testing: Toward Next-Generation Point-of-Care Diagnostics

Uyanga Ganbaatar et al.

Summary: As the COVID-19 pandemic continues, there is a need for a simple, rapid, early and sensitive point-of-care testing for the disease. CRISPR-based detection methods have gained attention for their simplicity, high sensitivity, and specificity, but face challenges for practical applications. These methods have the potential to become simpler, more reliable, more affordable, and faster, which is crucial for achieving point-of-care diagnostics.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

Article Hematology

Deciphering the mechanisms of CC-122 resistance in DLBCL via a genome-wide CRISPR screen

Zhongying Mo et al.

Summary: The study conducted genome-wide CRISPR/Cas9 screening and mechanistic characterization of CC-122 in DLBCL cell lines, identifying genes that regulate the response to CC-122, including both known and novel genetic factors.

BLOOD ADVANCES (2021)

Article Biochemistry & Molecular Biology

Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer

Jaclyn M. Einstein et al.

Summary: RNA-binding proteins (RBPs) play crucial roles in post-transcriptional gene expression regulation, and disrupting YTHDF2-dependent mRNA degradation can induce apoptosis in triple-negative breast cancer (TNBC) cells and tumors. Our study identified 57 RBP candidates with distinct functions in supporting MYC-driven oncogenic pathways, highlighting the therapeutic potential of RBPs, especially YTHDF2, in counteracting global mRNA synthesis in MYC-driven breast cancers.

MOLECULAR CELL (2021)

Article Multidisciplinary Sciences

In vivo genome-wide CRISPR screen reveals breast cancer vulnerabilities and synergistic mTOR/Hippo targeted combination therapy

Meiou Dai et al.

Summary: Through in vivo genome-wide CRISPR screening, tumor regulatory functions for components of the mTORC1/2 complex and YAP/Hippo pathway were identified in TNBC, showing that pharmacological inhibition of mTOR and YAP can reduce tumor growth in vivo.

NATURE COMMUNICATIONS (2021)

Article Multidisciplinary Sciences

High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer

Sarah E. Pierce et al.

Summary: The Spear-ATAC method enables simultaneous reading of chromatin accessibility profiles and sgRNA sequences from single cells, revealing the dynamic epigenetic responses to regulatory perturbations in cancer cells. Dynamics of transcription factor binding can drive epigenetic states, leading to a diversity of phenotypes.

NATURE COMMUNICATIONS (2021)

Article Biochemical Research Methods

Protocol A protocol for efficient CRISPR-Cas9-mediated knock-in in colorectal cancer patient-derived organoids

Takuya Okamoto et al.

Summary: The protocol involves generating genetically modified PDOs using the CRISPR-Cas9 system to knock-in the IRES-EGFP-P2A-iCaspase9 cassette into the 3' UTR of a potential cancer stem cell marker gene. This allows investigation into the potential for self-replication and pluripotency of these cells in stem cell research.

STAR PROTOCOLS (2021)

Article Multidisciplinary Sciences

Frequent loss of heterozygosity in CRISPR-Cas9-edited early human embryos

Gregorio Alanis-Lobato et al.

Summary: CRISPR-Cas9 genome editing shows promise for correcting disease-associated alleles in somatic cells, but studies in human embryos reveal challenges such as low mutation repair efficiency, high mosaicism, and unintended editing outcomes. Further research is crucial to evaluate the safety of genome editing techniques in human embryos.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Oncology

Modeling Breast Cancer Using CRISPR-Cas9-Mediated Engineering of Human Breast Organoids

Johanna F. Dekkers et al.

JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE (2020)

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Generalizable sgRNA design for improved CRISPR/Cas9 editing efficiency

Kasidet Hiranniramol et al.

BIOINFORMATICS (2020)

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Microsatellite instability: a review of what the oncologist should know

Kai Li et al.

CANCER CELL INTERNATIONAL (2020)

Article Biochemistry & Molecular Biology

In situ CRISPR-Cas9 base editing for the development of genetically engineered mouse models of breast cancer

Stefano Annunziato et al.

EMBO JOURNAL (2020)

News Item Multidisciplinary Sciences

CRISPR TREATMENT INSERTED DIRECTLY INTO BODY FOR THE FIRST TIME

Heidi Ledford

NATURE (2020)

Article Cell Biology

Programmable base editing of mutated TERT promoter inhibits brain tumour growth

Xinjian Li et al.

NATURE CELL BIOLOGY (2020)

Article Multidisciplinary Sciences

CRISPR-engineered T cells in patients with refractory cancer

Edward A. Stadtmauer et al.

SCIENCE (2020)

Article Biochemistry & Molecular Biology

Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies

Theodore L. Roth et al.

Article Genetics & Heredity

A Novel Hybrid CNN-SVR for CRISPR/Cas9 Guide RNA Activity Prediction

Guishan Zhang et al.

FRONTIERS IN GENETICS (2020)

Review Chemistry, Multidisciplinary

Innovative Precision Gene-Editing Tools in Personalized Cancer Medicine

Xiaofeng Dai et al.

ADVANCED SCIENCE (2020)

Article Chemistry, Multidisciplinary

Assay Techniques and Test Development for COVID-19 Diagnosis

Linda J. Carter et al.

ACS CENTRAL SCIENCE (2020)

Review Biotechnology & Applied Microbiology

Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors

Andrew V. Anzalone et al.

NATURE BIOTECHNOLOGY (2020)

Article Multidisciplinary Sciences

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Matthew H. Bailey et al.

NATURE COMMUNICATIONS (2020)

Article Biochemistry & Molecular Biology

Allele-Specific Chromosome Removal after Cas9 Cleavage in Human Embryos

Michael Zuccaro et al.

Article Education, Scientific Disciplines

Next-generation cell therapies: the emerging role of CAR-NK cells

Rafet Basar et al.

HEMATOLOGY-AMERICAN SOCIETY OF HEMATOLOGY EDUCATION PROGRAM (2020)

Article Biotechnology & Applied Microbiology

CRISPRi-based radiation modifier screen identifies long non-coding RNA therapeutic targets in glioma

S. John Liu et al.

GENOME BIOLOGY (2020)

Article Biochemistry & Molecular Biology

C-RNNCrispr: Prediction of CRISPR/Cas9 sgRNA activity using convolutional and recurrent neural networks

Guishan Zhang et al.

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL (2020)

Review Genetics & Heredity

Applications and advances of CRISPR-Cas9 in cancer immunotherapy

An-Liang Xia et al.

JOURNAL OF MEDICAL GENETICS (2019)

Article Multidisciplinary Sciences

WRN helicase is a synthetic lethal target in microsatellite unstable cancers

Edmond M. Chan et al.

NATURE (2019)

Article Biochemical Research Methods

In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens

Ryan D. Chow et al.

NATURE METHODS (2019)

Article Multidisciplinary Sciences

CRISPR-Cas9-mediated gene knockout in intestinal tumor organoids provides functional validation for colorectal cancer driver genes

Haruna Takeda et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

Article Multidisciplinary Sciences

Lineage tracing and targeting of IL17RB+ tuft cell-like human colorectal cancer stem cells

Norihiro Goto et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

Review Multidisciplinary Sciences

Cancer modeling meets human organoid technology

David Tuveson et al.

SCIENCE (2019)

Letter Oncology

Targeting glycosylation of PD-1 to enhance CAR-T cell cytotoxicity

Xiaojuan Shi et al.

JOURNAL OF HEMATOLOGY & ONCOLOGY (2019)

Article Multidisciplinary Sciences

Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning

Daqi Wang et al.

NATURE COMMUNICATIONS (2019)

Article Multidisciplinary Sciences

Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Beau R. Webber et al.

NATURE COMMUNICATIONS (2019)

Article Biochemistry & Molecular Biology

Immune checkpoint inhibitors win the 2018 Nobel Prize

Pei-Wei Huang et al.

BIOMEDICAL JOURNAL (2019)

Article Chemistry, Medicinal

Prediction of CRISPR sgRNA Activity Using a Deep Convolutional Neural Network

Li Xue et al.

JOURNAL OF CHEMICAL INFORMATION AND MODELING (2019)

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Human antibody-based chemically induced dimerizers for cell therapeutic applications

Zachary B. Hill et al.

NATURE CHEMICAL BIOLOGY (2018)

Article Biochemistry & Molecular Biology

Refined sgRNA efficacy prediction improves large- and small-scale CRISPR-Cas9 applications

Maurice Labuhn et al.

NUCLEIC ACIDS RESEARCH (2018)

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CRISPR-Cas9: A cornerstone for the evolution of precision medicine

Sleiman Razzouk

ANNALS OF HUMAN GENETICS (2018)

Article Biochemical Research Methods

Off-target predictions in CRISPR-Cas9 gene editing using deep learning

Jiecong Lin et al.

BIOINFORMATICS (2018)

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Gene Editing on Center Stage

Rasmus O. Bak et al.

TRENDS IN GENETICS (2018)

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Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes

Jasper Edgar Neggers et al.

NATURE COMMUNICATIONS (2018)

Article Engineering, Biomedical

Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs

Jennifer Listgarten et al.

NATURE BIOMEDICAL ENGINEERING (2018)

Article Biotechnology & Applied Microbiology

DeepCRISPR: optimized CRISPR guide RNA design by deep learning

Guohui Chuai et al.

GENOME BIOLOGY (2018)

Article Genetics & Heredity

High Activity Target-Site Identification Using Phenotypic Independent CRISPR-Cas9 Core Functionality

Laurence O. W. Wilson et al.

CRISPR JOURNAL (2018)

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Cancer CRISPR Screens In Vivo

Ryan D. Chow et al.

TRENDS IN CANCER (2018)

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Transcription control by the ENL YEATS domain in acute leukaemia

Michael A. Erb et al.

NATURE (2017)

Article Multidisciplinary Sciences

RNA targeting with CRISPR-Cas13

Omar O. Abudayyeh et al.

NATURE (2017)

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Simultaneous epitope and transcriptome measurement in single cells

Marlon Stoeckius et al.

NATURE METHODS (2017)

Article Biochemical Research Methods

Genetic interaction mapping in mammalian cells using CRISPR interference

Dan Du et al.

NATURE METHODS (2017)

Article Biochemical Research Methods

Pooled CRISPR screening with single-cell transcriptome readout

Paul Datlinger et al.

NATURE METHODS (2017)

Review Clinical Neurology

Human induced pluripotent stem cells for modelling neurodevelopmental disorders

Karthikeyan Ardhanareeswaran et al.

NATURE REVIEWS NEUROLOGY (2017)

Review Immunology

NK cell-based immunotherapy for cancer

Fang Fang et al.

SEMINARS IN IMMUNOLOGY (2017)

Review Biotechnology & Applied Microbiology

In Silico Meets In Vivo: Towarcs Computational CRISPR-Based sgRNA Design

Guo-hui Chuai et al.

TRENDS IN BIOTECHNOLOGY (2017)

Article Biochemical Research Methods

sgRNA Scorer 2.0: A Species-Independent Model To Predict CRISPR/Cas9 Activity

Raj Chari et al.

ACS SYNTHETIC BIOLOGY (2017)

Review Pediatrics

MLL-Rearranged Leukemias-An Update on Science and Clinical Approaches

Amanda C. Winters et al.

FRONTIERS IN PEDIATRICS (2017)

Article Genetics & Heredity

First-in-human Phase 1 CRISPR Gene Editing Cancer Trials: Are We Ready?

Francoise Baylis et al.

CURRENT GENE THERAPY (2017)

Article Biochemistry & Molecular Biology

Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq

Diego Adhemar Jaitin et al.

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CRISPR gene editing tested in a person

David Cyranoski

NATURE (2016)

News Item Multidisciplinary Sciences

First trial of CRISPR in people

David Cyranoski

NATURE (2016)

Article Multidisciplinary Sciences

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects

Benjamin P. Kleinstiver et al.

NATURE (2016)

Article Biotechnology & Applied Microbiology

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

John G. Doench et al.

NATURE BIOTECHNOLOGY (2016)

Review Oncology

Mechanism-driven biomarkers to guide immune checkpoint blockade in cancer therapy

Suzanne L. Topalian et al.

NATURE REVIEWS CANCER (2016)

Article Biotechnology & Applied Microbiology

Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

Maximilian Haeussler et al.

GENOME BIOLOGY (2016)

Review Pathology

Driver and Passenger Mutations in Cancer

Julia R. Pon et al.

ANNUAL REVIEW OF PATHOLOGY: MECHANISMS OF DISEASE, VOL 10 (2015)

Article Biochemistry & Molecular Biology

Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis

Sidi Chen et al.

Article Biochemistry & Molecular Biology

Identifying Drug-Target Selectivity of Small-Molecule CRM1/XPO1 Inhibitors by CRISPR/Cas9 Genome Editing

Jasper E. Neggers et al.

CHEMISTRY & BIOLOGY (2015)

Article Biochemistry & Molecular Biology

Sequence determinants of improved CRISPR sgRNA design

Han Xu et al.

GENOME RESEARCH (2015)

Article Biotechnology & Applied Microbiology

Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors

Xiaoling Wang et al.

NATURE BIOTECHNOLOGY (2015)

Article Biochemical Research Methods

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

Miguel A. Moreno-Mateos et al.

NATURE METHODS (2015)

Article Biochemical Research Methods

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

Raj Chari et al.

NATURE METHODS (2015)

Article Multidisciplinary Sciences

Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells

Hsin-Kai Liao et al.

NATURE COMMUNICATIONS (2015)

Article Biotechnology & Applied Microbiology

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Shengdar Q. Tsai et al.

NATURE BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases

Richard L. Frock et al.

NATURE BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system

Nathan Wong et al.

GENOME BIOLOGY (2015)

Article Biochemistry & Molecular Biology

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

Luke A. Gilbert et al.

Review Medicine, Research & Experimental

Expanding the genetic editing tool kit: ZFNs, TALENs, and CRISPR-Cas9

Rajat M. Gupta et al.

JOURNAL OF CLINICAL INVESTIGATION (2014)

Article Biotechnology & Applied Microbiology

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation

John G. Doench et al.

NATURE BIOTECHNOLOGY (2014)

Article Multidisciplinary Sciences

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

Ophir Shalem et al.

SCIENCE (2014)

Article Mathematical & Computational Biology

OMICtools: an informative directory for multi-omic data analysis

Vincent J. Henry et al.

DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION (2014)

Article Biotechnology & Applied Microbiology

Efficient genome editing in zebrafish using a CRISPR-Cas system

Woong Y. Hwang et al.

NATURE BIOTECHNOLOGY (2013)

Article Biochemical Research Methods

CRISPR RNA-guided activation of endogenous human genes

Morgan L. Maeder et al.

NATURE METHODS (2013)

Article Biochemical Research Methods

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

Ari E. Friedland et al.

NATURE METHODS (2013)

Article Multidisciplinary Sciences

RNA-Guided Human Genome Engineering via Cas9

Prashant Mali et al.

SCIENCE (2013)

Article Multidisciplinary Sciences

Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong et al.

SCIENCE (2013)

Article Biology

RNA-programmed genome editing in human cells

Martin Jinek et al.

Review Oncology

Immunotherapy of cancer in 2012

John M. Kirkwood et al.

CA-A CANCER JOURNAL FOR CLINICIANS (2012)

Article Multidisciplinary Sciences

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

Giedrius Gasiunas et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

Article Multidisciplinary Sciences

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

Elitza Deltcheva et al.

NATURE (2011)

Article Biochemistry & Molecular Biology

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

Rimantas Sapranauskas et al.

NUCLEIC ACIDS RESEARCH (2011)

Article Oncology

The biology and treatment of EML4-ALK non-small cell lung cancer

Takaaki Sasaki et al.

EUROPEAN JOURNAL OF CANCER (2010)

Article Multidisciplinary Sciences

CRISPR provides acquired resistance against viruses in prokaryotes

Rodolphe Barrangou et al.

SCIENCE (2007)

Article Biochemistry & Molecular Biology

Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements

FJM Mojica et al.

JOURNAL OF MOLECULAR EVOLUTION (2005)

Article Biochemistry & Molecular Biology

Identification of genes that are associated with DNA repeats in prokaryotes

R Jansen et al.

MOLECULAR MICROBIOLOGY (2002)