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JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 -, 期 -, 页码 -出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.2c11118
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In this study, a diverse repertoire of active, stable, and enantiodivergent fungal peroxidases was designed using the FuncLib algorithm based on phylogenetic and Rosetta calculations. Several designs showed unprecedented enantiodivergence, changing regioselectivity, and increasing catalytic efficiencies up to 10-fold, with improvements in total turnover numbers. Beneficial epistasis among the mutations and extensive reorganization of the heme channel contributed to this functional divergence. This work demonstrates the rapid design of highly functional peroxygenase libraries using FuncLib for various biotechnological applications, which are not naturally existing.
The generation of enantiodivergent biocatalysts for C-H oxyfunctionalizations is ever more important in modern synthetic chemistry. Here, we have applied the FuncLib algorithm based on phylogenetic and Rosetta calculations to design a diverse repertoire of active, stable, and enantiodivergent fungal perox-ygenases. 24 designs, each carrying 4-5 mutations in the catalytic core, were expressed functionally in yeast and benchmarked against characteristic model compounds. Several designs were active and stable in a range of temperature and pH, displaying unprecedented enantiodivergence, changing regioselectivity from alkyl to aromatic hydroxylation, and increasing catalytic efficiencies up to 10-fold, with 15-fold improvements in total turnover numbers over the parental enzyme. We find that this dramatic functional divergence stems from beneficial epistasis among the mutations and an extensive reorganization of the heme channel. Our work demonstrates that FuncLib can rapidly design highly functional libraries enriched in enantioselective peroxygenases not seen in nature for a range of biotechnological applications.
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