期刊
JOURNAL OF SURGICAL RESEARCH
卷 280, 期 -, 页码 371-378出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jss.2022.07.043
关键词
Chromogranin A; Distraction enterogenesis; Enteroendocrine; Ileum; Short bowel syndrome; Spring-lengthening
类别
资金
- National Institutes of Health [R01DK130972]
Intraluminal nitinol springs significantly lengthened porcine ileum, preserving its structure and function. The increase in density of enteroendocrine cells indicates enhanced endocrine function of the lengthened ileum.
Introduction: Short bowel syndrome is a devastating gastrointestinal disorder in which decreased bowel length results in inadequate absorption causing nutritional deficiencies. Current treatment options are accompanied by significant morbidity. We have proposed spring-mediated distraction enterogenesis as a method to lengthen bowel with success seen in porcine jejunum. We hypothesize that spring-mediated distraction enterogenesis can be demonstrated in porcine ileum with preservation of ileal structure and function.Materials and methods: Laparotomy was performed on juvenile female mini-Yucatan pigs and a gelatin-encapsulated compressed nitinol spring was inserted into the ileal lumen and affixed proximally and distally. A control segment distal to the spring segment was marked with sutures. Postoperatively, pigs were placed on a liquid diet and euthanized on postoperative day 7. Spring and control segments were measured and processed for immunohistochemistry to evaluate for the presence of vitamin B12-intrinsic factor cotransporter, chromogranin A-producing cells, and 5-HT producing cells.Results: All seven pigs survived to postoperative day 7 with no adverse effects. On average, pigs gained 84.3 +/- 66.4 g/d. Spring segments lengthened 1.5 +/- 0.7 cm with a relative lengthening by 128% +/- 56%, which was statistically significant when compared to control (P < 0.01). The average density of chromogranin-A cells in control compared to spring segments was not significantly changed (2.9 +/- 1.1 cells/mm versus 3.2 +/- 1.2 cells/mm, P = 0.17). Both vitamin B12-intrinsic factor cotransporter and 5-HT producing cells were present in both control and lengthened ileum.Conclusions: Intraluminal nitinol springs significantly lengthened porcine ileum. The in-crease in density of enteroendocrine cells may indicate enhanced endocrine function of the lengthened ileum.(c) 2022 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据