期刊
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
卷 79, 期 2, 页码 251-260出版社
SPRINGER
DOI: 10.1007/s13105-022-00935-z
关键词
Endothelium; Extracellular histones; Inflammation
This study reveals that extracellular histones can increase endothelial cell activation by increasing cytosolic reactive oxygen species production and triggering the overexpression of cell adhesion molecules. The study also identifies cyclooxygenase and NADPH oxidase as sources of reactive oxygen species production. These findings enhance our understanding of how extracellular histones contribute to systemic inflammatory responses in diseases.
Extracellular histones have been reported to aggravate different pathophysiological processes by increasing vascular permeability, coagulopathy, and inflammation. In the present study, we elucidate how extracellular histones (10-100 mu g/mL) concentration dependently increase cytosolic reactive oxygen species (ROS) production using human umbilical vein endothelial cells (HUVECs). Furthermore, we identify cyclooxygenase (COX) and NADPH oxidase (NOX) activity as sources of ROS production in extracellular histone-treated HUVEC. This COX/NOX-mediated ROS production is also involved in enhanced NF-kB activity and cell adhesion molecules (VCAM1 and ICAM1) expression in histone-treated HUVEC. Finally, by using different toll-like receptor (TLR) antagonists, we demonstrate the role of TLR4 in CAMs overexpression triggered by extracellular histones in endothelial cells. In conclusion, our data suggest that through TLR4 signaling, extracellular histones increase endothelial cell activation, a mechanism involving increased COX- and NOX-mediated ROS. These findings increase our understanding on how extracellular histones enhance systemic inflammatory responses in diseases in which histone release occurs as part of the pathological processes.
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