Red light photobiomodulation rescues murine brain mitochondrial respiration after acute hypobaric hypoxia

Low-level laser therapy, or photobiomodulation, utilizes red or near-infrared light for the treatment of patho-logical conditions due to the presence of intracellular photoacceptors, such as mitochondrial cytochrome c ox-idase, that serve as intermediates for the therapeutic effects. We present an in-detail analysis of the effect of low-intensity LED red light irradiation on the respiratory chain of brain mitochondria. We tested whether low-level laser therapy at 650 nm could alleviate the brain mitochondrial dysfunction in the model of acute hypobaric hypoxia in mice. The irradiation of the mitochondrial fraction of the left cerebral cortex with low-intensity LED red light rescued Complex I-supported respiration during oxidative phosphorylation, normalized the initial po-larization of the inner mitochondrial membrane, but has not shown any significant effect on the activity of Complex IV. In comparison, the postponed effect (in 24 h) of the similar transcranial irradiation following hypoxic exposure led to a less pronounced improvement of the mitochondrial functional state, but normalized respiration related to ATP production and membrane polarization. In contrast, the similar irradiation of the mitochondria isolated from control healthy animals exerted an inhibitory effect on CI-supported respiration. The obtained results provide significant insight that can be beneficial for the development of non-invasive phototherapy.

Automated summary

Low-level laser therapy using red or near-infrared light can improve mitochondrial dysfunction in the brain caused by hypoxia. The therapy was effective in improving respiration and membrane polarization, but had no significant effect on Complex IV activity. This research provides valuable insights for the development of non-invasive phototherapy.