High-resolution mapping of sensory fibers at the healthy and post-myocardial infarct whole transgenic hearts

The sensory nervous system is critical to maintain cardiac function. As opposed to efferent innervation, less is known about cardiac afferents. For this, we mapped the VGLUT2-expressing cardiac afferent fibers of spinal and vagal origin by using the VGLUT2::tdTomato double transgenic mouse as an approach to visualize the whole hearts both at the dorsal and ventral sides. For comparison, we colabeled mixed-sex transgenic hearts with either TUJ1 protein for global cardiac innervation or tyrosine hydroxylase for the sympathetic network at the healthy state or following ischemic injury. Interestingly, the nerve density for global and VGLUT2-expressing afferents was found significantly higher on the dorsal side compared to the ventral side. From the global nerve innervation detected by TUJ1 immunoreactivity, VGLUT2 afferent innervation was detected to be 15-25% of the total network. The detailed characterization of both the atria and the ventricles revealed a remarkable diversity of spinal afferent nerve ending morphologies of flower sprays, intramuscular endings, and end-net branches that innervate distinct anatomical parts of the heart. Using this integrative approach in a chronic myocardial infarct model, we showed a significant increase in hyperinnervation in the form of axonal sprouts for cardiac afferents at the infarct border zone, as well as denervation at distal sites of the ischemic area. The functional and physiological consequences of the abnormal sensory innervation remodeling post-ischemic injury should be further evaluated in future studies regarding their potential contribution to cardiac dysfunction.

Automated summary

The sensory nervous system is crucial for maintaining cardiac function. However, our understanding of cardiac afferents is limited. In this study, we mapped the VGLUT2-expressing cardiac afferent fibers and found that the dorsal side of the heart has higher nerve density compared to the ventral side. We also observed a diverse range of spinal afferent nerve endings that innervate different parts of the heart. In a chronic myocardial infarct model, we observed hyperinnervation at the infarct border zone and denervation at distal sites of the ischemic area. These findings highlight the importance of further studying the functional and physiological consequences of sensory innervation remodeling after ischemic injury.