Role of adenosine A2A receptors in the loss of consciousness induced by propofol anesthesia

The mechanism of propofol-anesthesia-induced loss of consciousness (LOC) remains largely unknown. We speculated that the adenosine A(2A) receptor serves as a vital molecular target in regulating LOC states under propofol anesthesia. c-Fos staining helped observe the changes in the neuronal activity in the nucleus accumbens (NAc). Initially, the adenosine signals in the NAc were measured under propofol anesthesia using fiber photometry recordings. Then, behavior tests and electrophysiological recordings were used to verify the effect of systemic A(2A)R agonist or antagonist treatment on propofol anesthesia. Next, the microinjection technique was used to clarify the role of the NAc A(2A)R under propofol anesthesia. Fiber photometry recordings were applied to assess the effect of A(2A)R agonist or antagonist systemic treatment on adenosine signal alterations in the NAc during propofol anesthesia. Then, as the GABAergic neurons are the main neurons in the NAc, we further measured the neuronal activity of GABAergic neurons. In our study, propofol anesthesia enhanced the neuronal activity in the NAc, and the adenosine signals were increased in the NAc. SCH58261 reduced the LOC time and sedative depth, while CGS21680 increased those via intraperitoneal injection. Additionally, CGS21680 increased the changes in delta, theta, alpha, beta, and low-gamma oscillations in the NAc. Moreover, microinjection of SCH58261 significantly shortened the LOC time, whereas microinjection of CGS21680 into the NAc significantly prolonged the LOC duration. The results illustrated that after A(2A)R agonist administration, the level of extracellular adenosine signals in the NAc was decreased and the neuronal activity of GABAergic neurons was enhanced, whereas after A(2A)R antagonist administration via intraperitoneal injection, the opposite occurred. This study reveals the vital role of the A(2A)R in propofol-induced LOC and that the A(2A)R could affect the maintenance of propofol anesthesia.

Automated summary

This study reveals the vital role of the adenosine A(2A) receptor (A(2A)R) in propofol-anesthesia-induced loss of consciousness (LOC). The adenosine signals in the nucleus accumbens (NAc) are regulated by A(2A)R and have an impact on the duration and depth of propofol anesthesia.