Magnetic poly(ε-caprolactone)-based nanocomposite membranes for bone cell engineering

Magnetic polymer nanocomposites manipulatable by an external magnetic field have a great potential for bone tissue regeneration. Various strategies have been proposed for their fabrication, as uniform distribution of magnetic nanoparticles (MNPs) within the polymer matrix remains to be the main challenge. The aim of this study was to design poly(epsilon-caprolactone) (PCL) matrix containing iron oxide nanoparticles stabilized by satu-rated fatty acids with the increasing number of carbon atoms, i.e., caprylic (CA), palmitic (PA), and stearic acids (SA). We investigated the effect of the type of fatty acid and the content of particles in nanocomposite mem-branes on their physicochemical properties and biological response toward the SAOS-2 cells. The incorporation of the MNPs in PCL matrix resulted in a gradual increase of Young's modulus, a slight decrease of tensile strength, and a significant decrease of stress and strain at break. The addition of SA-stabilized MNPs (1 wt%) in the PCL matrix increased its strength. The membranes containing CA-stabilized MNPs remained non-toxic to-wards SAOS-2 cells, while the cytotoxicity of other nanocomposites increased with the enhanced length of carbon chains of fatty acids stabilizing MNPs, as well as with their increasing content in membranes.

Automated summary

Magnetic polymer nanocomposites that can be manipulated by an external magnetic field have great potential for bone tissue regeneration. In this study, a poly(epsilon-caprolactone) (PCL) matrix containing iron oxide nanoparticles stabilized by various saturated fatty acids was designed. The addition of stearic acid-stabilized magnetic nanoparticles increased the strength of the composite material, while the addition of caprylic acid-stabilized nanoparticles showed no toxicity.