Genome-scale angiosperm phylogenies based on nuclear, plastome, and mitochondrial datasets

Angiosperms dominate the Earth's ecosystems and provide most of the basic necessities for human life. The major angiosperm clades comprise 64 orders, as recognized by the APG IV classification. However, the phylogenetic relationships of angiosperms remain unclear, as phylogenetic trees with different topologies have been reconstructed depending on the sequence datasets utilized, from targeted genes to transcriptomes. Here, we used currently available de novo genome data to reconstruct the phylogenies of 366 angiosperm species from 241 genera belonging to 97 families across 43 of the 64 orders based on orthologous genes from the nuclear, plastid, and mitochondrial genomes of the same species with compatible datasets. The phylogenetic relationships were largely consistent with previously constructed phylogenies based on sequence variations in each genome type. However, there were major inconsistencies in the phylogenetic relationships of the five Mesangiospermae lineages when different genomes were examined. We discuss ways to address these inconsistencies, which could ultimately lead to the reconstruction of a comprehensive angiosperm tree of life. The angiosperm phylogenies presented here provide a basic framework for further updates and comparisons. These phylogenies can also be used as guides to examine the evolutionary trajectories among the three genome types during lineage radiation.

Automated summary

In this study, the phylogenies of 366 angiosperm species belonging to 97 families and 43 orders were reconstructed based on orthologous genes from the nuclear, plastid, and mitochondrial genomes. The results were largely consistent with previous studies, but there were major discrepancies in the phylogenetic relationships of the five Mesangiospermae lineages when different genomes were examined. The findings provide a basic framework for further research and can be used to explore the evolutionary trajectories of the three genome types.