期刊
JOURNAL OF IMMUNOLOGY
卷 209, 期 10, 页码 1960-1972出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.2200543
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资金
- National Institutes of Health (NIH) National Heart, Lung, and Blood Institute [R01HL140837]
- NIH National Institute of Arthritis and Musculoskeletal and Skin Diseases [R01AR072212]
- Children's Discovery Institute of Washington University
- St. Louis Children's Hospital
- National Institute of Allergy and Infectious Diseases [5R01AI150669-01A1]
- National Institute of General Medical Sciences [R35GM118027-01]
- NIH Shared Instrumentation Grant [S10 RR027552]
This study investigates the relative roles of macrophage and neutrophil NOX2 in the host response to Aspergillus fumigatus. The results show that macrophage NOX2 limits germination of inhaled A. fumigatus conidia, while reduced neutrophil NOX2 enhances inflammation to fungal cell walls and promotes fungal growth.
Aspergillus fumigatus is an important opportunistic fungal pathogen and causes invasive pulmonary aspergillosis in conditions with compromised innate antifungal immunity, including chronic granulomatous disease, which results from inherited deficiency of the superoxide-generating leukocyte NADPH oxidase 2 (NOX2). Derivative oxidants have both antimicrobial and immunoregulatory activity and, in the context of A. fumigatus, contribute to both fungal killing and dampening inflammation induced by fungal cell walls. As the relative roles of macrophage versus neutrophil NOX2 in the host response to A. fumigatus are incompletely understood, we studied mice with conditional deletion of NOX2. When NOX2 was absent in alveolar macrophages as a result of LysM-Cre-mediated deletion, germination of inhaled A. fumigatus conidia was increased. Reducing NOX2 activity specifically in neutrophils via S100a8 (MRP8)-Cre also increased fungal burden, which was inversely proportional to the level of neutrophil NOX2 activity. Moreover, diminished NOX2 in neutrophils synergized with corticosteroid immunosuppression to impair lung clearance of A. fumigatus. Neutrophil-specific reduction in NOX2 activity also enhanced acute inflammation induced by inhaled sterile fungal cell walls. These results advance understanding into cell-specific roles of NOX2 in the host response to A. fumigatus. We show that alveolar macrophage NOX2 is a nonredundant effector that limits germination of inhaled A. fumigatus conidia. In contrast, reducing NOX2 activity only in neutrophils is sufficient to enhance inflammation to fungal cell walls as well as to promote invasive A. fumigatus. This may be relevant in clinical settings with acquired defects in NOX2 activity due to underlying conditions, which overlap risk factors for invasive aspergillosis.
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