Performance and mechanisms of biochar-assisted vermicomposting in accelerating di-(2-ethylhexyl) phthalate biodegradation in farmland soil

Biochar and earthworms can accelerate di-(2-ethylhexyl) phthalate (DEHP) degradation in soils. However, little is known regarding the effect of biochar-assisted vermicomposting on soil DEHP degradation and the underlying mechanisms. Therefore, the present study investigated DEHP degradation performance and bacterial community changes in farmland soils using earthworms, biochar, or their combination. Biochar-assisted vermicomposting significantly improved DEHP degradation through initial physical adsorption on biochar and subsequent rapid biodegradation in the soil, earthworm gut, and charosphere. Burkholderiaceae, Pseudomonadaceae, and Flavobacteriaceae were the potential DEHP degraders and were enriched in biochar-assisted vermicomposting. In particularly, Burkholderiaceae and Sphingomonadaceae were enriched in the earthworm gut and charosphere, possibly explaining the mechanism of accelerated DEHP degradation in biochar-assisted vermicomposting. Soil pH, soil organic matter, and humus (humic acid, fulvic acid, and humin) increased by earthworms or biochar enhanced DEHP degradation. These findings imply that biochar-assisted vermicomposting enhances DEHP removal not only through rapid physical sorption but also through the improvement of soil physicochemical characteristics and promotion of degraders in the soil, earthworm gut, and charosphere. Overall, biochar-assisted vermicomposting is a suitable method for the remediation of organic-contaminated farmland soils.

Automated summary

Biochar and earthworms can accelerate the degradation of DEHP in soils. The present study investigated the effect of biochar-assisted vermicomposting on DEHP degradation and the bacterial community in farmland soils. Biochar-assisted vermicomposting significantly improved DEHP degradation through physical adsorption on biochar and subsequent biodegradation in the soil, earthworm gut, and charosphere. Burkholderiaceae, Pseudomonadaceae, and Flavobacteriaceae were the potential DEHP degraders enriched in biochar-assisted vermicomposting. Factors such as soil pH, organic matter, and humus were also found to affect DEHP degradation.