Preparation and characterization of glucoamylase microcapsules prepared by W/O/W type complex coacervation freeze drying

Glucoamylase was often used in the brewing industry but was unstable to several environmental factors and reacted quickly to produce fermentable sugar, which limited its applications. Microencapsulation could effectively overcome the drawbacks. This study evaluated the feasibility of the preparation of glucoamylase microcapsules (GM) using W/O/W complex coacervation-freeze-drying method. The parameters of the microcapsules were optimized by the response surface optimization design: core-wall ratio at 1:1, wall-material concentration at 8%, and coagulation time for 20 min. Under current condition, the final microencapsulation efficiency reached 85.64 +/- 1.60%. Glucoamylase could be slowly released for more than 96 h in the liquid state, and could react slowly for more than 336 h in the solid state. The optimized GM were incubated for 1 h, and the relative enzyme activity was better than that of free glucoamylase under high temperature conditions. The water capacity, solubility, morphology, differential scanning calorimetry, and Fourier transform infrared spectroscopy were conducted. Glucoamylase exhibited good sustained release characteristics. The microcapsules were more resistant to environmental stimuli and showed stronger robustness after optimization. Practical ApplicationSaccharification enzymes are often used in the winemaking industry, and direct addition will cause the fermentation process to heat up too quickly, resulting in the inactivation of microorganisms and saccharification enzymes, affecting the efficiency of saccharification enzymes. Therefore, microcapsules are used to encapsulate the saccharification enzyme, and its efficacy is slowly released for a long time during the fermentation process.

Automated summary

This study successfully prepared glucoamylase microcapsules (GM) using the W/O/W complex coacervation-freeze-drying method. The optimized GM showed a high encapsulation efficiency of 85.64% and exhibited sustained release characteristics in both liquid and solid states. The microcapsules also demonstrated higher enzyme activity under high temperature conditions. Overall, this research provides important theoretical and practical guidance for the application of glucoamylase in the brewing industry.