期刊
JOURNAL OF FLUORESCENCE
卷 33, 期 3, 页码 923-932出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-022-03129-4
关键词
Chromenoquinoline; Methylation; Fluorescent probe; Nucleic acid; Lipid droplet
Two chromenoquinoline-based fluorescent probes 1a-b were synthesized and studied, showing good photostability and selectivity after 6 h irradiation, strong absorption and emission in organic solvents, and the influence of alkylation on the optical properties of 1b.
Two chromenoquinoline-based fluorescent probes 1a-b have been synthesized and investigated. Photofading behaviors of compounds 1a-b showed that at least 89% absorption remained after 6 h irradiating, meanwhile, many of ions and amino acids had negligible impacts on their fluorescence intensity, which meant they had excellent photostability and selectivity. Probes 1a-b exhibited strong absorption and emission in organic solvents with large fluorescence quantum yields, even in water probe 1a still had a relatively large fluorescence quantum yield (20%). Combined with DFT calculation, the influence of alkylation on optical properties of 1b was elucidated. In addition, the fluorescence intensity of probe 1b with red emission enhanced by 5.4-fold and 5.3-fold after DNA and RNA added, and the fluorescence quantum yield increased from 3% to 17% and 14%, respectively, but the neutral molecule 1a had no response to nucleic acid. Furthermore, confocal microscopy imaging of probes 1a-b showed that 1a targeted lipid droplets while the methylated probe 1b to nucleus in living HeLa cells. The results indicated that the subcellular targeting zone could be changed by alkylation of nitrogen atom on chromenoquinoline-based conveniently, which provided a new idea for designing and synthesizing new subcellular labeled probes.
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