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Rubisco, the imperfect winner: it's all about the base

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Resurrected Rubisco suggests uniform carbon isotope signatures over geologic time

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Summary: The earliest geochemical indicators of microbes-and the enzymes that powered them-can be traced back to approximately 3.8 billion years ago on Earth. However, the assumption that the behaviors of extant microbes and enzymes are consistent with their predecessors contradicts our understanding of the inherent variability of living systems. In this study, the researchers examined the uniformitarian assumption for an enzyme called RuBisCO, which is thought to generate carbon isotope indicators of biological activity. They resurrected a Precambrian-age RuBisCO by engineering its ancient DNA inside a cyanobacterium genome and measured its fitness and carbon-isotope-discrimination profile. The results suggest that Precambrian uniformitarian assumptions may be valid, but with important caveats. Experimental studies that explore the molecular foundations of life's earliest traces are crucial.

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Structural plasticity enables evolution and innovation of RuBisCO assemblies

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Summary: Oligomerization is a core structural feature of many proteins, but there is a lack of diversity-driven structural studies on the evolutionary trajectory of these assemblies. This study reveals the evolutionary history of RuBisCOs and discovers a new tetrameric RuBisCO, demonstrating how structural plasticity gives rise to new oligomeric states.

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Summary: Membraneless organelles containing Rubisco enzyme are common in organisms using CO2 concentrating mechanisms for photosynthetic carbon acquisition. The Rubisco condensates in different organisms, like carboxysomes in cyanobacteria and proteobacteria, and pyrenoids in some algae and hornworts, enhance CO2 fixation compared to free enzyme. Mathematical models suggest that the enhanced function of carboxysomes is due to elevated CO2 levels generated within the organelle via colocalized carbonic anhydrase and inwardly diffusing bicarbonate ions.

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