Mesoporous silica nanoparticles adsorb aflatoxin B1 and reduce mycotoxin-induced cell damage

The present study examined the effects of mesoporous silica nanoparticles (MSNs) on its adsorption capacity of aflatoxin B-1 (AFB(1)). Moreover, the study evaluated the toxicity of MSNs with AFB(1) using NIH3T3 cells and hemolysis test. The obtained MSNs were spherical, irregular-like in shape, having a mean size of 39.97 +/- 7.85 nm and a BET surface area of 1195 m(2)/g. At 0.1 mg mL(-1) concentration of MSN, the AFB(1) adsorption capacity was 30%, which reached 70% when the MSN concentration increased to 2.0 mg mL(-1). Our findings showed that AFB(1) was adsorbed (similar to 67%) in the first few minutes on being in contact with MSNs, reaching an adsorption capacity of similar to 70% after 15 min. Thereafter, the adsorption capacity remained constant in solution, demonstrating that the MSNs adsorbed toxins even beyond overnight. MSN treatment (0.5-2.0 mg mL(-1)) using NIH3T3 cells did not result in any reduction in cell viability. In addition, MSN treatment completely reversed the cytotoxic effect of AFB(1) at all concentrations. Hemolysis test also revealed no hemolysis in MSNs evaluated alone and in those combined with AFB(1). To the best of our knowledge, this study is the first to demonstrate that MSN can reduce cell toxicity produced by AFB(1) due to its potential to adsorb mycotoxins.

Automated summary

The study investigated the adsorption capacity of mesoporous silica nanoparticles (MSNs) on aflatoxin B-1 (AFB(1)), as well as the toxicity of MSNs with AFB(1) using NIH3T3 cells and hemolysis test. The results showed that MSNs had a high adsorption capacity for AFB(1), with an increase in adsorption when the MSN concentration increased. MSNs also demonstrated the ability to reduce cell toxicity produced by AFB(1) and showed no hemolysis in combination with AFB(1).