Immobilization of exopolymeric substances from bacteria for metal removal: A study on characterization, optimization, reusability and toxicity

This study investigated the immobilization of exopolymeric substances (EPS) from Bacillus cereus using sodium alginate to form EPS beads for metal removal. The EPS beads were characterized and their optimum biosorption conditions established (biosorbent dosage, initial metal concentration and pH of metal solutions). The EPS beads were also tested for reusability by using them continuously for five metal removal cycles with desorption process in between cycles. The toxicity of the treated metal solutions was tested by phytotoxicity tests. Results revealed that EPS beads demonstrated significantly higher metal removal efficiency (Pb: 99.26%, Cr: 50.73%, Cu: 48.94%, Zn: 29.81%, Cd: 20.29%) compared to plain alginate beads (without EPS) (Pb: 84.45%, Cu: 31%, Cr: 28.37%, Zn: 11.91%, Cd: 9.37%). SEM-EDX analysis detected Cu, Pb, Zn, Cd and Cr on the surface of EPS beads. Optimum conditions for Pb removal by EPS beads were from the use of 0.1 g of biosorbent at 100 mg/L initial metal concentration and pH 5. By contrast, Cu, Zn, Cd and Cr were optimally removed by 0.3 g of biosorbent at 25 mg/ L initial metal concentration and pH 5. EPS beads can be reused up to five times while maintaining a high rate of metal removal efficiency (Pb-99.52%, Cr-89.23%, Cu-89.17%, Zn-52.52%, Cd-39.12%). This was achieved through desorption with nitric acid that consistently recovered 76-93% of the metal adsorbed. FTIR analysis reveals that nitric acid is capable of restoring the functional groups present within EPS beads, allowing it to bind with metal ions in repeated cycles. Metal solutions treated with EPS beads were less toxic as seedling shoots (pre-treated: 0-10 cm, post-treated: 1.2-18.1 cm) and roots (pre-treated: 0-7.8 cm, post-treated: 0.8-15.1 cm) grew well, which suggested that reduced levels of metals led to reduced phytotoxicity. This study provides an insight into the use of EPS beads for metal removal, highlighting the benefits and reusability of the beads for future wastewater treatment.

Automated summary

This study investigated the use of sodium alginate to immobilize exopolymeric substances (EPS) from Bacillus cereus in the form of EPS beads for metal removal. The EPS beads demonstrated significantly higher metal removal efficiency compared to plain alginate beads, and they could be reused multiple times while maintaining a high rate of metal removal efficiency. The treatment with EPS beads resulted in reduced phytotoxicity in the metal solutions.