Targeted CuFe2O4 hybrid nanoradiosensitizers for synchronous chemoradiotherapy

Multifunctional nanoplatforms based on novel bimetallic nanoparticles have emerged as effective radio -sensitizers owing to their potential capability in cancer cells radiosensitization. Implementation of chemotherapy along with radiotherapy, known as synchronous chemoradiotherapy, can augment the treatment efficacy. Herein, a tumor targeted nanoradiosensitizer with synchronous chemoradiotion properties, termed as CuFe2O4@BSA-FA-CUR, loaded with curcumin (CUR) and modified by bovine serum albumin (BSA) and folic acid (FA) was developed to enhance tumor accumulation and promote the anti-cancer activity while attenuating adverse effects. Both copper (Cu) and iron (Fe) were utilized in the construction of these submicron scale entities, therefore strong radiosensitization effect is anticipated by implementation of these two metals. The structur-e-function relationships between constituents of nanomaterials and their function led to the development of nanoscale materials with great radiosensitizing capacity and biosafety. BSA was used to anchor Fe and Cu ions but also to improve colloidal stability, blood circulation time, biocompatibility, and further functionalization. Moreover, to specifically target tumor sites and enhance cellular uptake, FA was conjugated onto the surface of hybrid bimetallic nanoparticles. Finally, CUR as a natural chemotherapeutic agent was encapsulated into the developed bimetallic nanoparticles. With incorporation of all abovementioned stages into one multifunctional nanoplatform, CuFe2O4@BSA-FA-CUR is produced for synergistic chemoradiotherapy with positive outcomes. In vitro investigation revealed that these nanoplatforms bear excellent biosafety, great tumor cell killing ability and radiosensitizing capacity. In addition, high cancer-suppression efficiency was observed through in vivo studies. It is worth mentioning that co-use of CuFe2O4@BSA-FA-CUR nanoplatforms and X-ray radiation led to complete tumor ablation in almost all of the treated mice. No mortality or radiation-induced normal tissue toxicity were observed following administration of CuFe2O4@BSA-FA-CUR nanoparticles which highlights the biosafety of these submicron scale entities. These results offer powerful evidence for the potential capability of CuFe2O4@BSA-FA-CUR in radiosensitization of malignant tumors and opens up a new avenue of research in this area.

Automated summary

This article introduces a novel bimetallic nanoparticle-based multifunctional nanoplatform that acts as an effective radiosensitizer and enhances treatment efficacy by combining chemotherapy and radiotherapy. By encapsulating CUR into CuFe2O4 nanoparticles modified by BSA and FA, tumor accumulation is increased and adverse effects are reduced. The nanoplatform exhibits excellent biosafety, tumor-killing ability, and radiosensitizing capacity in vitro, as well as high cancer suppression efficiency in vivo. The co-administration of CuFe2O4@BSA-FA-CUR nanoparticles with X-ray radiation leads to complete tumor ablation in treated mice without any mortality or radiation-induced normal tissue toxicity, highlighting their biosafety and potential for radiosensitization of malignant tumors.