Facial nerve axotomy induces morphological changes in hippocampal pyramidal neurons

Facial nerve injury in rats have been widely used to study functional and structural changes that occur in the injured motoneurons and other central nervous system structures related with sensorimotor processing. A decrease in long-term potentiation of hippocampal CA3-to-CA1 commissural synapse has recently been reported related to this peripheral injury. Additionally, it has been found increased corticosterone plasmatic levels, impairment in spatial memory consolidation, and hippocampal microglial activation in animals with facial nerve axotomy. In this work, we analyzed the neuronal morphology of hippocampal CA1 and CA3 pyramidal neurons in animals with either reversible or irreversible facial nerve injury. For this purpose, brain tissues of injured animals sacrificed at different postlesion times, were stained with the Golgi-Cox method and compared with control brains. It was found that both reversible and irreversible facial nerve injury-induced significant decreases in dendritic tree complexity, dendritic length, branch points, and spine density of hippocampal neurons. However, such changes' timing varied according to hippocampal area (CA1 vs. CA3), dendritic area (apical vs. basal), and lesion type (reversible vs. irreversible). In general, the observed changes were transient when animals had the possibility of motor recovery (reversible injury), but perdurable if the recovery from the lesion was impeded (irreversible injury). CA1 apical and CA3 basal dendritic tree morphology were more sensible to irreversible injury. It is concluded that facial nerve injury induced significant changes in hippocampal CA1 and CA3 pyramidal neurons morphology, which could be related to LTP impairments and microglial activation in the hippocampal formation, previously described.

Automated summary

Facial nerve injury has been widely used in rats to study the changes in motoneurons and other central nervous system structures related to sensorimotor processing. Recent studies have found that this injury is associated with a decrease in long-term potentiation, increased corticosterone levels, impairment in spatial memory consolidation, and hippocampal microglial activation. In this study, the neuronal morphology of hippocampal pyramidal neurons in rats with reversible or irreversible facial nerve injury was analyzed. The results showed significant decreases in dendritic tree complexity, dendritic length, branch points, and spine density of hippocampal neurons in both types of injuries, but the timing and sensitivity varied depending on the hippocampal area, dendritic area, and lesion type.