4.6 Article

Capillary gel electrophoresis of very high molecular weight glycoproteins. Commercial and tailor-made gels for analysis of human monomeric and secretory immunoglobulin A

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Chemistry, Analytical

N-glycosylation structure - function characterization of omalizumab, an anti-asthma biotherapeutic product

Miklos Szabo et al.

Summary: Omalizumab, a glycoprotein based biotherapeutics, is widely used to improve asthma symptoms and reduce corticosteroid usage. The glycosylation of such drugs plays a crucial role in their effectiveness and clearance time, with comprehensive analysis needed during production and release.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2022)

Article Polymer Science

Capillary Sodium Dodecyl Sulfate Agarose Gel Electrophoresis of Proteins

Daniel Sarkozy et al.

Summary: This paper introduces a new capillary agarose gel electrophoresis method for the separation of low molecular weight immunoglobulin subunits. Through adjusting the concentration of agarose and boric acid, the sieving performance of the gel was optimized, achieving good resolution and separation power. Additionally, this method does not require capillary regeneration steps, saving analysis time.
Article Biochemical Research Methods

Four-step approach to efficiently develop capillary gel electrophoresis methods for viral vaccine protein analysis

Lars Geurink et al.

Summary: Efficient method development is crucial for the analysis of vaccine proteins. By identifying critical method parameters and establishing a set of steps based on these parameters, a four-step approach was successfully used for the development of two CE methods for viral vaccine protein analysis.

ELECTROPHORESIS (2021)

Article Chemistry, Analytical

Capillary sodium dodecyl sulfate gel electrophoresis of proteins: Introducing the three dimensional Ferguson method

Csenge Filep et al.

Summary: This study utilized capillary SDS gel electrophoresis with dextran and borate cross-linking to analyze the electrophoretic process and molecular characteristics of SDS-protein complexes with the help of 3D Ferguson plots and KR plots, revealing the impact of dextran and borate concentrations on separation characteristics during electrophoresis.

ANALYTICA CHIMICA ACTA (2021)

Article Chemistry, Analytical

Effect of the Monomer Cross-Linker Ratio on the Separation Selectivity of Monoclonal Antibody Subunits in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis

Csenge Filep et al.

Summary: This study investigated the impact of the monomer and cross-linker ratio on the analysis of therapeutic monoclonal antibodies using SDS-CGE, leading to the development of a retention model for better separation selectivity understanding. Introduction of three-dimensional selectivity plots offered an easy separation optimization option for the separation problem in hand.

ANALYTICAL CHEMISTRY (2021)

Article

Top 15 Best-Selling Drugs Launched in 2020

Alex Philippidis

Genetic Engineering & Biotechnology News (2021)

Article Chemistry, Analytical

Fundamentals of Capillary Electrophoretic Migration and Separation of SDS Proteins in Borate Cross-Linked Dextran Gels

Andras Guttman et al.

Summary: Recent advancements in protein therapeutics necessitate high-resolution bioseparation techniques, such as SDS-CGE using a borate cross-linked dextran matrix. By adjusting the dextran and borate concentration ratios, optimal separation for specific biopharmaceutical modalities can be achieved with excellent reproducibilities.

ANALYTICAL CHEMISTRY (2021)

Review Immunology

Diversity and dynamism of IgA-microbiota interactions

Kelsey E. Huus et al.

Summary: Intestinal IgA plays a crucial role in maintaining the balance of commensal microbiota and protecting against harmful pathogens. Factors influencing IgA-microbiota interactions include host, bacterial, and environmental factors. Recent advances have shed light on the antigenic specificity of IgA and the impact of diet and nutrition on microbiota recognition.

NATURE REVIEWS IMMUNOLOGY (2021)

Article Biochemical Research Methods

Enhancement of covalent aggregate quantification of protein therapeutics by non-reducing capillary gel electrophoresis using sodium hexadecyl sulfate (CE-SHS)

Qian Guan et al.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2020)

Article Multidisciplinary Sciences

Robust and Specific Secretory IgA Against SARS-CoV-2 Detected in Human Milk

Alisa Fox et al.

ISCIENCE (2020)

Editorial Material Biotechnology & Applied Microbiology

The Increasingly Human and Profitable Monoclonal Antibody Market

Antonio L. Grilo et al.

TRENDS IN BIOTECHNOLOGY (2019)

Article

Top 15 Best-Selling Drugs of 2018

Alex Philippidis

Genetic Engineering & Biotechnology News (2019)

Article Medicine, Research & Experimental

Production, characterization, and in vivo half-life extension of polymeric IgA molecules in mice

T. Noelle Lombana et al.

Review Immunology

IgA: Structure, Function, and Developability

Patricia de Sousa-Pereira et al.

ANTIBODIES (2019)

Article Obstetrics & Gynecology

Storage of Unfed and Leftover Mothers' Own Milk

April D. Fogleman et al.

BREASTFEEDING MEDICINE (2018)

Review Immunology

Structure and function relationships in IgA

J. M. Woof et al.

MUCOSAL IMMUNOLOGY (2011)

Article Biochemical Research Methods

Use of immunodotting to select the desorption agent for immunochromatography

A Puerta et al.

JOURNAL OF IMMUNOLOGICAL METHODS (2004)