4.6 Article

Development of a unified gradient theory for ion-pair chromatography using oligonucleotide separations as a model case

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biochemical Research Methods

Building machine-learning-based models for retention time and resolution predictions in ion pair chromatography of oligonucleotides

Martin Enmark et al.

Summary: Support vector regression models accurately predict the retention times of oligonucleotides separated using gradient ion-pair chromatography. The results show that the variation in retention time is dependent on the sequence, with sequences having high guanine-cytosine content and a terminal C being more difficult to reach critical resolution.

JOURNAL OF CHROMATOGRAPHY A (2022)

Article Biochemical Research Methods

Effect of ion-pairing reagent hydrophobicity on liquid chromatography and mass spectrometry analysis of oligonucleotides

Michael Donegan et al.

Summary: We systematically studied the performance of thirteen alkylamines as ion-pairing reagents for ion-pair reversed-phase liquid chromatography (IP RP LC) and proposed a method to classify the hydrophobicity of alkylamines. The results showed that alkylamines with higher hydrophobicity allowed for effective separation of oligonucleotides at lower buffer concentrations, and replacing acetic acid with hexafluoroisopropanol improved the mass spectrometry signal.

JOURNAL OF CHROMATOGRAPHY A (2022)

Article Biochemical Research Methods

Selectivity limits of and opportunities for ion pair chromatographic separation of oligonucleotides

Martin Enmark et al.

Summary: In this study, it was investigated how oligonucleotide retention and selectivity in ion pair chromatography are affected by electrostatic and non-electrostatic interactions. It was found that selectivity increases with increasing surface potential and analyte charge difference for oligonucleotides of different charges. Additionally, selectivity for analytes of the same charge can be increased by decreasing co-solvent volume fraction or temperature.

JOURNAL OF CHROMATOGRAPHY A (2021)

Article Biochemical Research Methods

Analytical and preparative separation of phosphorothioated oligonucleotides: columns and ion-pair reagents

Martin Enmark et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2020)

Review Chemistry, Analytical

Characterization of therapeutic oligonucleotides by liquid chromatography

Alexandre Goyon et al.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2020)

Review Biotechnology & Applied Microbiology

Advances in oligonucleotide drug delivery

Thomas C. Roberts et al.

NATURE REVIEWS DRUG DISCOVERY (2020)

Article Biochemical Research Methods

Impact of stationary-phase pore size on chromatographic performance using oligonucleotide separation as a model

Joakim Bagge et al.

JOURNAL OF CHROMATOGRAPHY A (2020)

Review Medicine, Research & Experimental

Targeting RNA: A Transformative Therapeutic Strategy

Wei Yin et al.

CTS-CLINICAL AND TRANSLATIONAL SCIENCE (2019)

Review Biochemical Research Methods

Chromatographic approaches for the characterization and quality control of therapeutic oligonucleotide impurities

N. M. El Zahar et al.

BIOMEDICAL CHROMATOGRAPHY (2018)

Article Biochemistry & Molecular Biology

Impurities in Oligonucleotide Drug Substances and Drug Products

Daniel Capaldi et al.

NUCLEIC ACID THERAPEUTICS (2017)

Review Pharmacology & Pharmacy

RNA Targeting Therapeutics: Molecular Mechanisms of Antisense Oligonucleotides as a Therapeutic Platform

C. Frank Bennett et al.

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY (2010)

Article Biochemical Research Methods

Martin-Synge algorithm for the solution of equilibrium-dispersive model of liquid chromatography

Krisztian Horvath et al.

JOURNAL OF CHROMATOGRAPHY A (2010)

Article Biochemical Research Methods

Enantioseparation of flurbiprofen on amylose-derived chiral stationary phase by supercritical fluid chromatography

Chen Wenda et al.

JOURNAL OF CHROMATOGRAPHY A (2009)

Review Biochemical Research Methods

Influence of mobile phase acid-base equilibria on the chromatographic behaviour of protolytic compounds

M Rosés et al.

JOURNAL OF CHROMATOGRAPHY A (2002)

Article Biochemical Research Methods

The measurement and meaning of void volumes in reversed-phase liquid chromatography

CA Rimmer et al.

JOURNAL OF CHROMATOGRAPHY A (2002)