期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 27, 期 3, 页码 435-445出版社
WILEY
DOI: 10.1111/jcmm.17670
关键词
immunocompetent; induced pluripotent stem cell; microglia; retinal organoids
Microglia are important immune cells in the retina, regulating neuronal survival and synaptic pruning. Researchers co-cultured retinal organoids with microglia-like cells and found that the organoids developed normally and retained their function. This immunocompetent in vitro retinal model provides a more relevant platform for studying the human retina.
Microglia are the primary resident immune cells in the retina. They regulate neuronal survival and synaptic pruning making them essential for normal development. Following injury, they mediate adaptive responses and under pathological conditions they can trigger neurodegeneration exacerbating the effect of a disease. Retinal organoids derived from human induced pluripotent stem cells (hiPSCs) are increasingly being used for a range of applications, including disease modelling, development of new therapies and in the study of retinogenesis. Despite many similarities to the retinas developed in vivo, they lack some key physiological features, including immune cells. We engineered an hiPSC co-culture system containing retinal organoids and microglia-like (iMG) cells and tested their retinal invasion capacity and function. We incorporated iMG into retinal organoids at 13 weeks and tested their effect on function and development at 15 and 22 weeks of differentiation. Our key findings showed that iMG cells were able to respond to endotoxin challenge in monocultures and when co-cultured with the organoids. We show that retinal organoids developed normally and retained their ability to generate spiking activity in response to light. Thus, this new co-culture immunocompetent in vitro retinal model provides a platform with greater relevance to the in vivo human retina.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据