期刊
JOURNAL OF AUTOIMMUNITY
卷 133, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jaut.2022.102901
关键词
Spondyloarthritis; HLA-B*27; T cell receptor; Clonal T cell expansions; Transcriptomics; mRNAseq
类别
资金
- German Research Foundation (DFG) [DO 420/4, PO 2124/2-1, EXC 2145 SyNergy, 390857198]
- Charite-Universitatsmedizin Berlin
- Berlin Institute of Health
This study investigated the T cell receptor (TCR) usage and whole transcriptomes of CD8+ single cells to analyze the pathogenesis of Spondyloarthritis (SpA). The findings suggest that (auto)antigenic peptides may play a role in the development of SpA.
Spondyloarthritis (SpA) is a chronic inflammatory disease that is tightly linked to HLA-B*27 but the patho-physiological basis of this link is still unknown. It is discussed whether either the instability of HLA-B*27 molecules triggers predominantly innate immune reactions or yet unknown antigenic peptides presented by HLA-B*27 induce adaptive autoimmune reactions by CD8+ T cells. To analyze the pathogenesis of SpA, we here investigated the T cell receptor (TCR) usage and whole transcriptomes of CD8+ single cells from synovial fluid of HLA-B*27-positive SpA patients and HLA-B*27-negative controls. In HLA-B*27-positive patients, we confirmed preferential expression of several TCR beta-chain families, found even more restricted usage of particular TCR alpha-chains, assigned matching TCR alpha beta-chain pairs with homologous CDR3-sequences, and detected identical TCR-chains in different patients. Gene expression analyses by single cell mRNAseq revealed that genes specific for the tissue resident memory phenotype, exhaustion, and apoptosis were particularly highly expressed in expanded clonotypes from HLA-B*27-positive SpA patients. Together, several independent lines of evidence argue in favor of an (auto)antigenic peptide related pathogenesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据