期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 70, 期 49, 页码 15499-15508出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c06791
关键词
bioavailability; CD36; fatty acid amide hydrolase; lymphatic vessel; oleamide; portal vein
资金
- Food Science Institute Foundation (Ryoushoku-kenkyukai)
- Japan Society for the Promotion of Science [2019A12]
- Nagaoka Co. Ltd. [20K21285]
This study provides insights into the transport form and pathway of oleamide, as well as its absorption and metabolism properties. Oleamide is primarily transported via the portal vein as a complex with albumin. It is taken up by CD36 in the small intestine and degraded intracellularly by FAAH.
This study aimed to obtain information on the transport form and pathway from the intestine to the peripheral tissues and on the intestinal absorption and metabolism properties of oleamide (cis-9-octadecenamide). Oleamide was primarily transported via the portal vein. Density gradient centrifugation indicated that plasma oleamide was enriched in the fractions containing albumin in the portal and peripheral blood. Oleamide formed a complex with albumin in an endothermic reaction (apparent K-d = 4.4 mu M). The CD36 inhibitor inhibited the oleamide uptake into the intestinal epithelial Caco-2 cells, and oleamide decreased the cell surface CD36 level. The fatty acid amide hydrolase (FAAH) inhibitor increased the transepithelial transport of oleamide across Caco-2 cells and the plasma oleamide concentration in mice intragastrically administered with oleamide. These results indicate that oleamide is transported primarily via the portal vein as a complex with albumin. Furthermore, we suggest that oleamide is taken up via CD36 in the small intestine and degraded intracellularly by FAAH.
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