期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 -, 期 -, 页码 2943-2951出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c08153
关键词
glycosylated astaxanthin; metabolic engineering; CBFD; HBFD; Yarrowia lipolytica; Adonis aestivalis
In this study, glycosylated astaxanthin was successfully produced in yeast for the first time. Through modular assembly and expression regulation, the yield of astaxanthin was increased by 4.9-fold. Finally, introduction of the crtX gene resulted in the highest reported yield of microbially produced glycosylated astaxanthin (1.47 mg/L).
Astaxanthin is a high-value red pigment and antioxidant widely used in the pharmaceutical, cosmetic, and food industries. However, the hydrophobicity of astaxanthin causes its low bioavailability. Glycosylation can substantially increase the water solubility of astaxanthin, thus enhancing its bioavailability, photostability, and biological activities. In this study, we report for the first time the heterologous production of glycosylated astaxanthin in Yarrowia lipolytica. By appropriate removal of the chloroplast transit peptide, carotenoid 4-hydroxy-beta-ring 4-dehydrogenase (HBFD) and carotenoid beta-ring 4-dehydrogenase (CBFD) from Adonis aestivalis were expressed in a beta-carotene-producing Y. lipolytica strain, resulting in astaxanthin production with a yield of 0.59 mg/L, 0.05 mg/g DCW. This is the first time to successfully construct a plant-derived astaxanthin synthesis pathway in yeast. Modularized assembly of CBFD and HBFD, replacement of the promoter upstream CBFD, increasing the precursor beta-carotene supply, and regulating the expressions of CBFD and HBFD led to a 4.9-fold increase in astaxanthin production (3.46 mg/L). Finally, introduction of crtX from Pantoea ananatis ATCC 19321 into the astaxanthin-producing strain enabled glycosylated astaxanthin production, and the yield reached 1.47 mg/L, which is the highest yield of microbially produced glycosylated astaxanthin reported to date.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据