4.7 Article

Circuit-wide proteomics profiling reveals brain region-specific protein signatures in the male WKY rats with endogenous depression

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JOURNAL OF AFFECTIVE DISORDERS
卷 320, 期 -, 页码 98-107

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ELSEVIER
DOI: 10.1016/j.jad.2022.09.086

关键词

Depression; Wistar Kyoto rats; Proteomics; Medial prefrontal cortex; Nucleus accumbens; Hippocampus

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This study investigates the molecular mechanisms underlying the genetic susceptibility to depression in the WKY rat model. Comparison with Wistar rats reveals significant differences in depression-like behaviors and synaptic plasticity in the WKY strain. Proteomic analysis identifies brain region-specific differentially-expressed proteins that closely correlate with depression-like phenotypes. These findings provide novel insights into the pathogenesis of depression in males.
Background: Although the Wistar Kyoto (WKY) rat has been consistently recognized as an animal model with endogenous depression, the exact molecular mechanisms underlying its genetic susceptibility to depression remain undetermined.Methods: Compared with the Wistar rats, the depression-like behaviors of the male WKY ones were evaluated by both the sucrose preference test and forced swimming test. Golgi staining analysis was conducted to access the dendritic morphology. TMT-labelled quantitative proteomics analyses were respectively performed in the medial prefrontal cortex (mPFC), nucleus accumbens (NAc), and hippocampus (Hip), followed by KEGG enrichment -based clustering analysis, Venn diagram analysis, and Pearson correlation analysis.Results: The WKY strain showed significant differences in both the depression-like behaviors and synaptic plasticity. Moreover, the WKY model displayed markedly distinct differentially-expressed protein (DEP) profiles, with minor differences between the WKY subgroups. A cerebral regional commonality and specificity were evident in the signaling pathways enriched in the WKY model, and a total of 15 brain region-specific DEPs were identified to closely correlate with the depression-like phenotypes (in the mPFC: Lrrc8d, Dcun1d2, and Mtnd5; in the NAc: Ccdc154, Sec14l2, Kif2a, LOC680322, Me1, Mknk1, and Ret7; in the Hip: Sec14l2, Serpinf2, LOC103694855, Fam13c, and Loxl1). Data were available via ProteomeXchange with identifier PXD029079.Limitations: Female WKY rats are not included, and the roles of these candidate DEPs in depression remain further elucidation.Conclusion: The present study further evidences the brain region-specific protein signatures in the male WKY model with endogenous depression, providing novel insights into the pathogenesis of depression in males.

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