4.7 Article

Potato Stu-miR398b-3p Negatively Regulates Cu/Zn-SOD Response to Drought Tolerance

期刊

出版社

MDPI
DOI: 10.3390/ijms24032525

关键词

stu-miR398b-3p; Cu; Zn SOD; drought resistance; expression analysis

向作者/读者索取更多资源

One of the main impacts of drought stress on plants is the excessive accumulation of reactive oxygen species (ROS), which can be highly toxic to cells if not cleared quickly. Superoxide dismutase (SOD) is a crucial antioxidant enzyme that plays the first role in scavenging active oxygen. The miR398s family of miRNAs has been shown to be significant in plants' response to environmental stress. Potato stu-miR398b-3p was found to cleave Cu/Zn SOD mRNA, and overexpression of stu-miR398b-3p led to decreased SOD activity during drought stress.
One of the main impacts of drought stress on plants is an excessive buildup of reactive oxygen species (ROS). A large number of center dot OH, highly toxic to cells, will be produced if too much ROS is not quickly cleared. At the heart of antioxidant enzymes is superoxide dismutase (SOD), which is the first antioxidant enzyme to function in the active oxygen scavenging system. To shield cells from oxidative injury, SOD dismutation superoxide anion free radicals generate hydrogen peroxide and molecule oxygen. Cu/Zn SOD is a kind of SOD antioxidant enzyme that is mostly found in higher plants' cytoplasm and chloroplasts. Other studies have demonstrated the significance of the miR398s family of miRNAs in the response of plants to environmental stress. The cleavage location of potato stu-miR398b-3p on Cu/Zn SOD mRNA was verified using RLM-5 ' RACE. Using the potato variety 'Desiree', the stu-miR398b-3p overexpression mutant was created, and transgenic lines were raised. SOD activity in transgenic lines was discovered to be decreased during drought stress, although other antioxidant enzyme activities were mostly unaltered. Transgenic plants will wilt more quickly than wild-type plants without irrigation. Additionally, this demonstrates that the response of Cu/Zn SOD to drought stress is adversely regulated by potato stu-miR398b-3p.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据