Potato Stu-miR398b-3p Negatively Regulates Cu/Zn-SOD Response to Drought Tolerance

One of the main impacts of drought stress on plants is an excessive buildup of reactive oxygen species (ROS). A large number of center dot OH, highly toxic to cells, will be produced if too much ROS is not quickly cleared. At the heart of antioxidant enzymes is superoxide dismutase (SOD), which is the first antioxidant enzyme to function in the active oxygen scavenging system. To shield cells from oxidative injury, SOD dismutation superoxide anion free radicals generate hydrogen peroxide and molecule oxygen. Cu/Zn SOD is a kind of SOD antioxidant enzyme that is mostly found in higher plants' cytoplasm and chloroplasts. Other studies have demonstrated the significance of the miR398s family of miRNAs in the response of plants to environmental stress. The cleavage location of potato stu-miR398b-3p on Cu/Zn SOD mRNA was verified using RLM-5 ' RACE. Using the potato variety 'Desiree', the stu-miR398b-3p overexpression mutant was created, and transgenic lines were raised. SOD activity in transgenic lines was discovered to be decreased during drought stress, although other antioxidant enzyme activities were mostly unaltered. Transgenic plants will wilt more quickly than wild-type plants without irrigation. Additionally, this demonstrates that the response of Cu/Zn SOD to drought stress is adversely regulated by potato stu-miR398b-3p.

Automated summary

One of the main impacts of drought stress on plants is the excessive accumulation of reactive oxygen species (ROS), which can be highly toxic to cells if not cleared quickly. Superoxide dismutase (SOD) is a crucial antioxidant enzyme that plays the first role in scavenging active oxygen. The miR398s family of miRNAs has been shown to be significant in plants' response to environmental stress. Potato stu-miR398b-3p was found to cleave Cu/Zn SOD mRNA, and overexpression of stu-miR398b-3p led to decreased SOD activity during drought stress.