The Histone Chaperone Network Is Highly Conserved in Physarum polycephalum

The nucleosome is composed of histones and DNA. Prior to their deposition on chromatin, histones are shielded by specialized and diverse proteins known as histone chaperones. They escort histones during their entire cellular life and ensure their proper incorporation in chromatin. Physarum polycephalum is a Mycetozoan, a clade located at the crown of the eukaryotic tree. We previously found that histones, which are highly conserved between plants and animals, are also highly conserved in Physarum. However, histone chaperones differ significantly between animal and plant kingdoms, and this thus probed us to further study the conservation of histone chaperones in Physarum and their evolution relative to animal and plants. Most of the known histone chaperones and their functional domains are conserved as well as key residues required for histone and chaperone interactions. Physarum is divergent from yeast, plants and animals, but PpHIRA, PpCABIN1 and PpSPT6 are similar in structure to plant orthologues. PpFACT is closely related to the yeast complex, and the Physarum genome encodes the animal-specific APFL chaperone. Furthermore, we performed RNA sequencing to monitor chaperone expression during the cell cycle and uncovered two distinct patterns during S-phase. In summary, our study demonstrates the conserved role of histone chaperones in handling histones in an early-branching eukaryote.

Automated summary

The nucleosome is made up of histones and DNA, and histones are protected by specialized proteins called histone chaperones before they are deposited onto chromatin. In this study, the conservation of histone chaperones in Physarum polycephalum and their evolution relative to animals and plants were investigated. It was found that while histones are highly conserved in Physarum, histone chaperones differ significantly between animal and plant kingdoms. RNA sequencing revealed distinct patterns of chaperone expression during the cell cycle in Physarum. Overall, this study demonstrates the conserved role of histone chaperones in an early-branching eukaryote.