Functional Characterization of the GNAT Family Histone Acetyltransferase Elp3 and GcnE in Aspergillus fumigatus

Post-translational modifications of chromatin structure by histone acetyltransferase (HATs) play a pivotal role in the regulation of gene expression and diverse biological processes. However, the function of GNAT family HATs, especially Elp3, in the opportunistic human pathogenic fungus Aspergillus fumigatus is largely unknown. To investigate the roles of the GNAT family HATs Elp3 and GcnE in the A. fumigatus, we have generated and characterized individual null Delta elp3 and Delta gcnE mutants. The radial growth of fungal colonies was significantly decreased by the loss of elp3 or gcnE, and the number of asexual spores (conidia) in the Delta gcnE mutant was significantly reduced. Moreover, the mRNA levels of the key asexual development regulators were also significantly low in the Delta gcnE mutant compared to wild type (WT). Whereas both the Delta elp3 and Delta gcnE mutants were markedly impaired in the formation of adherent biofilms, the Delta gcnE mutant showed a complete loss of surface structure and of intercellular matrix. The Delta gcnE mutant responded differently to oxidative stressors and showed significant susceptibility to triazole antifungal agents. Furthermore, Elp3 and GcnE function oppositely in the production of secondary metabolites, and the Delta gcnE mutant showed attenuated virulence. In conclusion, Elp3 and GcnE are associated with diverse biological processes and can be potential targets for controlling the pathogenic fungus.

Automated summary

Post-translational modifications of chromatin structure by histone acetyltransferase (HATs) are crucial for gene expression regulation and various biological processes. The function of GNAT family HATs, especially Elp3, in the opportunistic human pathogenic fungus Aspergillus fumigatus remains largely unknown. In this study, we investigated the roles of Elp3 and GcnE in A. fumigatus by generating and characterizing individual null Delta elp3 and Delta gcnE mutants. The results showed that Elp3 and GcnE are associated with diverse biological processes and can be potential targets for controlling the pathogenic fungus.