期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 24, 页码 -出版社
MDPI
DOI: 10.3390/ijms232415527
关键词
selenium nanoparticles; LCS-SeNPs; chitosan; characterization; colitis
资金
- Key R & D Program of Zhejiang Province
- Basic Scientific Research Funds of Zhejiang Provincial Universities
- National Key Research and Development Program
- Science and Technology Project of Zhoushan
- [2022C02043]
- [2022C02059]
- [2021JZ007]
- [2020YFD0900900]
- [2021YFD2100504]
- [2020C21014]
- [2020C21018]
- [2020C21047]
- [2021C13044]
In this study, a new form of selenium nanoparticle, LCS-SeNPs, was synthesized and characterized. The results showed that LCS-SeNPs protected against colon injury and inflammation, improved intestinal barrier dysfunction, and alleviated oxidative stress. Moreover, LCS-SeNPs achieved these effects through the regulation of NF-kappa B and Nrf2 signaling pathways.
Selenium nanoparticles have attracted extensive attention due to their good bioavailability and activity. In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid. The size, element state, morphology and elementary composition of LCS-SeNPs were characterized by using various spectroscopic and microscopic measurements. The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction and the inherent mechanisms of this process were investigated. The results showed that LCS-SeNPs, with an average diameter of 198 nm, zero-valent and orange-red relatively uniform spherical particles were prepared. LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se. LCS-SeNPs reduced colon injury and inflammation symptoms and improved intestinal barrier dysfunction. LCS-SeNPs significantly reduced serum and colonic inflammatory cytokines TNF-alpha and IL-6 levels. Moreover, LCS-SeNPs remarkably increased antioxidant enzyme GSH-Px levels in serum and colonic tissue. Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-kappa B signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway. Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
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