期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/ijms24010417
关键词
germinal vesicle oocyte; in vitro maturation; cryopreservation; embryo development; vitrification; survival
This study explores the impact of in vitro maturation (IVM) medium and vitrification on the survival and development of germinal vesicle (GV) oocytes obtained from stimulated cycles. The results show that a higher percentage of oocytes reach the MII stage if they are vitrified before undergoing IVM. However, the significance of these differences depends on the medium used and sample size. Similar survival rates and development to blastocysts were observed in all conditions studied.
How does the in vitro maturation (IVM) medium and the vitrification procedure affect the survival of germinal vesicle (GV) oocytes obtained from stimulated cycles and their development to the blastocyst stage? In total, 1085 GV human oocytes were obtained after women underwent a cycle of controlled ovarian stimulation, and these oocytes were subjected to IVM before or after their vitrification. IVM was carried out in two commercial culture media not specifically designed for maturation. MII oocytes were then activated and embryo development until day 6 was evaluated. According to the results, a higher percentage of oocytes reach the MII stage if they are vitrified before they undergo IVM. Nevertheless, the medium used and the sample size determine whether these differences become significant or not. Similar survival rates and development to blastocysts were observed in all the conditions studied.
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