期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/ijms232214122
关键词
inflammatory cytokines; IL-15; IL-21; naive CD8(+) T cells; TCR; cytokine priming; ATACseq; gene expression
资金
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2014-04692]
Cytokine priming enhances antigen responsiveness and functional fitness of naive CD8(+) T cells by modulating chromatin accessibility. This study reveals the molecular mechanisms underlying cytokine priming and its impact on gene expression, suggesting its significance in immune response and regulation.
Naive CD8(+) T lymphocytes exposed to certain inflammatory cytokines undergo proliferation and display increased sensitivity to antigens. Such 'cytokine priming' can promote the activation of potentially autoreactive and antitumor CD8(+) T cells by weak tissue antigens and tumor antigens. To elucidate the molecular mechanisms of cytokine priming, naive PMEL-1 TCR transgenic CD8(+) T lymphocytes were stimulated with IL-15 and IL-21, and chromatin accessibility was assessed using the assay for transposase-accessible chromatin (ATAC) sequencing. PMEL-1 cells stimulated by the cognate antigenic peptide mgp100(25-33) served as controls. Cytokine-primed cells showed a limited number of opening and closing chromatin accessibility peaks compared to antigen-stimulated cells. However, the ATACseq peaks in cytokine-primed cells substantially overlapped with those of antigen-stimulated cells and mapped to several genes implicated in T cell signaling, activation, effector differentiation, negative regulation and exhaustion. Nonetheless, the expression of most of these genes was remarkably different between cytokine-primed and antigen-stimulated cells. In addition, cytokine priming impacted the expression of several genes following antigen stimulation in a synergistic or antagonistic manner. Our findings indicate that chromatin accessibility changes in cytokine-primed naive CD8(+) T cells not only underlie their increased antigen responsiveness but may also enhance their functional fitness by reducing exhaustion without compromising regulatory controls.
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