期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 23, 页码 -出版社
MDPI
DOI: 10.3390/ijms232314769
关键词
frontotemporal dementia; amyotrophic lateral sclerosis; TDP-43; proteinopathy; stress granules
资金
- National Health and Medical Research Council of Australia (NHMRC) Program
- NHMRC [APP1132524]
- [APP1154692]
- [GNT2008066]
The use of cellular models in drug discovery for ALS and FTD is common, but there is currently no consensus on the most accurate model to replicate key pathological features. This study characterized two TDP-43 proteinopathy cellular models and found that different effects were observed when small molecule probes were exposed to these models. The study highlights the challenges of using cellular models in lead development and emphasizes the need for evaluations of novel therapeutics across various cell lines and aetiological models.
The use of cellular models is a common means to investigate the potency of therapeutics in pre-clinical drug discovery. However, there is currently no consensus on which model most accurately replicates key aspects of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) pathology, such as accumulation of insoluble, cytoplasmic transactive response DNA-binding protein (TDP-43) and the formation of insoluble stress granules. Given this, we characterised two TDP-43 proteinopathy cellular models that were based on different aetiologies of disease. The first was a sodium arsenite-induced chronic oxidative stress model and the second expressed a disease-relevant TDP-43 mutation (TDP-43 M337V). The sodium arsenite model displayed most aspects of TDP-43, stress granule and ubiquitin pathology seen in human ALS/FTD donor tissue, whereas the mutant cell line only modelled some aspects. When these two cellular models were exposed to small molecule chemical probes, different effects were observed across the two models. For example, a previously disclosed sulfonamide compound decreased cytoplasmic TDP-43 and increased soluble levels of stress granule marker TIA-1 in the cellular stress model without impacting these levels in the mutant cell line. This study highlights the challenges of using cellular models in lead development during drug discovery for ALS and FTD and reinforces the need to perform assessments of novel therapeutics across a variety of cell lines and aetiological models.
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