Blood Plasma Proteome: A Meta-Analysis of the Results of Protein Quantification in Human Blood by Targeted Mass Spectrometry

A meta-analysis of the results of targeted quantitative screening of human blood plasma was performed to generate a reference standard kit that can be used for health analytics. The panel included 53 of the 296 proteins that form a stable part of the proteome of a healthy individual; these proteins were found in at least 70% of samples and were characterized by an interindividual coefficient of variation <40%. The concentration range of the selected proteins was 10(-10)-10(-3) M and enrichment analysis revealed their association with rare familial diseases. The concentration of ceruloplasmin was reduced by approximately three orders of magnitude in patients with neurological disorders compared to healthy volunteers, and those of gelsolin isoform 1 and complement factor H were abruptly reduced in patients with lung adenocarcinoma. Absolute quantitative data of the individual proteome of a healthy and diseased individual can be used as the basis for personalized medicine and health monitoring. Storage over time allows us to identify individual biomarkers in the molecular landscape and prevent pathological conditions.

Automated summary

A meta-analysis was conducted to create a reference standard kit for health analytics by conducting targeted quantitative screening of human blood plasma. The kit includes 53 proteins that are stably present in the proteome of a healthy individual and found in at least 70% of samples, with an interindividual coefficient of variation <40%. These proteins, with concentration range of 10(-10)-10(-3) M, were associated with rare familial diseases based on enrichment analysis. The study also identified specific protein concentration changes in patients with neurological disorders and lung adenocarcinoma compared to healthy volunteers.