期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/ijms232214106
关键词
Listeria monocytogenes; internalin A; PMSC; primers
资金
- Ministero della Salute [E56C18001780001, PRC2018008]
This study presents a new set of primers for sequencing inlA in both lineages. The presence of premature stop-codon mutations in inlA is correlated with attenuated virulence, and lineage I isolates are most frequently associated with clinical cases. Using these newly designed primers, the presence of inlA PMSCs in food, food processing environments, and clinical isolates was assessed.
Listeria monocytogenes is a major human foodborne pathogen responsible for listeriosis. The virulence factor Internalin A (inlA) has a key role in the invasion of L. monocytogenes into the human intestinal epithelium, and the presence of premature stop-codons (PMSC) mutations in the inlA gene sequence is correlated with attenuated virulence. The inlA sequencing process is carried out by dividing the gene into three sections which are then reassembled to obtain the full gene. The primers available however were only able to entirely amplify the lineage II isolates. In this study, we present a set of new primers which allow inlA sequencing of isolates belonging to both lineages, since lineage I isolates are the ones most frequently associated to clinical cases. Using newly designed primers, we assessed the presence of inlA PMSCs in food, food processing environments and clinical isolates.
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