期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 223, 期 -, 页码 231-239出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.10.276
关键词
Aptamer; Colorimetric detection; Lipopolysaccharide; Endotoxin; Gold nanoparticle; SYBR Green
资金
- National Natural Science Foundation of China
- National Key Research and Development Program of China
- [32001785]
- [2021YFA0716704]
A colorimetric assay based on LPS binding aptamer and gold nanoparticles was developed for rapid and accurate detection of LPS concentration, which contributes to the protection of food safety and human health.
Lipopolysaccharide (LPS) poses a considerable threat to food safety and human health. A colorimetric assay for LPS detection based on LPS binding aptamer (LBA) and SYBR Green I (SG) mediated aggregation of gold nanoparticles (AuNPs) was established. In the absence of LPS, the LBA was absorbed onto the AuNPs surface which prevented SG-induced aggregation of AuNPs, and the sensing system exhibited red color. When LPS was added, it interacted with the LBA, forming a complex. At higher LPS concentration, many LBAs were exhausted resulting in SG-induced aggregation of AuNPs, and color change from red to blue. The range of colorimetric detection of LPS was linear in 0-12 EU/mL, with a limit of detection of 0.1698 EU/mL. Spiked LPS in real samples and interfering substances were also identified. This assay ingeniously using the fluorescent dye SG as an effective trigger of AuNPs aggregation, is rapid and facile than most of those earlier reported LBA-based LPS assays, and there is potential to be modified to construct assays for other targets.
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