Green synthesis of silica-coated magnetic nanocarriers for simultaneous purification-immobilization of 8-1,3-xylanase

Tailoring magnetic nanocarriers with tunable properties is of great significance for the development of multi-functional candidate materials in numerous fields. Herein, we report a one-pot biomimetic silicification-based method for the synthesis of silica-coated magnetic nanoparticles. The synthesis process was mild, low cost, and highly efficient, which took only about 21 min compared with 4.5-120 h in other literature. Then, the carriers had been characterized by VSM, SEM, TEM, XRD, FT-IR, and EDS to confirm their function. To evaluate the usefulness of the carriers, they were adopted to couple the purification and immobilization of 8-1,3-xylanase from the cell lysate in a single step with high immobilization yield (92.8 %) and high activity recovery (82.4 %). The immobilized enzyme also retained 58.4 % of the initial activity after 10 cycles and displayed good storage properties, and improved thermal stability, which would be promising in algae biomass bioconversion as well as other diverse applications.

Automated summary

Tailoring magnetic nanocarriers with tunable properties using a biomimetic silicification-based method is reported in this study. The synthesis process was mild, low cost, and highly efficient, taking only about 21 minutes compared to other literature which took 4.5-120 hours. The carriers were characterized using various techniques and were found to have high immobilization yield and activity recovery when used for purification and immobilization of 8-1,3-xylanase. The immobilized enzyme demonstrated good stability and retained a significant amount of initial activity after multiple cycles, making it promising for algae biomass bioconversion and other applications.