期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 227, 期 -, 页码 925-937出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.12.172
关键词
Chitosan; Tumor acidity-responsive nanomicelles; dePEGylation
To enhance the antitumor potency of doxorubicin (DOX), researchers developed tumor acidity-responsive polymeric nanomicelles from PEGylated chitosan-g-PLGA conjugates. These nanomicelles showed robust stability and optimized drug release in weak acidic environments, leading to improved cellular internalization and enhanced anticancer effect of DOX. In vivo experiments demonstrated that these nanomicelles effectively suppressed tumor growth, highlighting their potential in improving DOX chemotherapy.
To effectively promote antitumor potency of doxorubicin (DOX), a regularly used chemotherapy drug, the tumor acidity-responsive polymeric nanomicelles from self-assembly of the as-synthesized amphiphilic benzoic iminecontaining PEGylated chitosan-g-poly(lactic-co-glycolic acid) (PLGA) conjugates were developed as vehicles of DOX. The attained PEGylated chitosan-g-PLGA nanomicelles with high PEGylation degree (H-PEG-CSPNs) were characterized to exhibit a onion-like core-shell-corona structure consisting of a hydrophobic PLGA core covered by benzoic imine-rich chitosan shell and outer hydrophilic PEG corona. The DOX-carrying H-PEG-CSPNs (DOX@H-PEG-CSPNs) displayed robust colloidal stability under large-volume dilution condition and in a serumcontaining aqueous solution of physiological salt concentration. Importantly, the DOX@H-PEG-CSPNs in weak acidic milieu undergoing the hydrolysis of benzoic imine bonds and increased protonation of chitosan shell showed dePEGylation and surface charge conversion. Also, the considerable swelling of protonated chitosan shell within DOX@H-PEG-CSPNs accelerated drug release. Notably, the cellular internalization of DOX@H-PEGCSPNs by TRAMP-C1 prostate cancer cells under mimic acidic tumor microenvironment was efficiently boosted upon acidity-triggered detachment of PEG corona and exposure of positively-charged chitosan shell, thus augmenting DOX-mediated anticancer effect. Compared to free DOX molecules, the DOX@H-PEG-CSPNs appreciably suppressed TRAMP-C1 tumor growth in vivo, thereby showing great promise in improving DOX chemotherapy.
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